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Plant Receptor Kinases: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1621
ISBN13: 9781493970629
Publisher: Humana Pr
Published: Jun 1 2017
Pages: 196
Weight: 1.29
Height: 0.50 Width: 7.00 Depth: 10.00
Language: English
PART I Expression and Purification of PRKs
1. Expression of Plant Receptor Kinases in E. coli
Moneeza Akbar Agha, David Lightfoot, and Ahmed Jawaad Afzal
2. Expression of Plant Receptor Kinases in Yeast
MarÃa Laura Barberini and Jorge Muschietti
3. Expression of Plant Receptor Kinases in Tobacco BY-2 Cells
Hidefumi Shinohara and Yoshikatsu Matsubayashi
4. Cell-free Synthesis of Plant Receptor Kinases
Akira Nozawa, Keiichirou Nemoto, Shunsuke Nomura, Satoshi Yamanaka, Kohki Kido, and Tatsuya Sawasaki
5. Purification of Plant Receptor Kinases from Plant Plasma Membranes
Jin Suk Lee
PART II Ligand Binding and Activation of PRKs
6. Photoaffinity Labeling of Plant Receptor Kinases
Hidefumi Shinohara and Yoshikatsu Matsubayashi
7. Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases
Markus Albert and Ursula Fürst
PART III In silico Methods for Identification and Analyses of PRKs
8. Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis
Aleksia Vaattovaara, Jarkko Salojärvi, and Michael Wrzaczek
9. In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping
Russell J. S. Orr and Reidunn B. Aalen
PART IV Identification of Enzymatic Functions and Interacting Partners of PRKs
10. Co-immunoprecipitation of Plant Receptor Kinases
MarÃa Laura Barberini and Jorge Muschietti
11. Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems
Keiichirou Nemoto, Akira Nozawa, Satoshi Yamanaka, Shunsuke Nomura, Kohki Kido, and Tatsuya Sawasaki
12. Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners
Matthew R Meyer, Shweta Shah, and A. Gururaj Rao
13. In vitro Assessment of Guanylyl Cyclase Activity of Plant Receptor Kinases
Misjudeen Raji and Chris Gehring
14. BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases
Cornelia Fischer, Margret Sauter, and Petra Dietrich
15. Pull Down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases
Christine Kaufmann and Margret Sauter
PART V Imaging of PRK Dynamics
16. The use of FRET/FLIM to Study Proteins Interacting with Plant Receptor KinasesStefanie Weidtkamp-Peters and Yvonne Stahl
17. Detection and Analyses of Endocytosis of Plant Receptor Kinases
Jenna Loiseau and Silke Robatzek
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