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Plant Proteases and Plant Cell Death: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2447
ISBN13: 9781071620786
Publisher: Humana Pr
Published: May 19 2022
Pages: 299
Weight: 1.67
Height: 0.75 Width: 7.00 Depth: 10.00
Language: English
1 Expression and purification of the type I metacaspase from a cryptophyte Guillardia theta, GtMCA-I
Vida Strancar, Katarina Petra van Midden, Marina Klemenčič, Christiane Funk
2 Expression and purification of the type II metacaspase from a unicellular green alga Chlamydomonas reinhardtii
Igor Sabljic, Yong Zou, Marina Klemenčič, Christiane Funk, Jerry Ståhlberg, Peter Bozhkov
3 Expression in Escherichia coli, refolding, and purification of plant aspartic proteases
Pedro Castanheira, Carla Almeida, Daniela Dias-Pedroso, Isaura Simões
4 Production of functional plant legumain proteases using the Leishmania tarentolae expression system
Elfriede Dall, Andreas Licht, Hans Brandstetter
5 Purification of His-tagged proteases from the apoplast of agroinfiltrated N. benthamiana
Mariana Schuster, Judith K. Paulus, Jiorgos Kourelis, Renier A. L. van der Hoorn
6 Identification of cognate protease/substrate pairs by use of class-specific inhibitors
Annick Stintzi, Nils Stührwohldt, Stefanie Royek, Andreas Schaller
7 Peptide backbone modifications for the assessment of cleavage site relevance in precursors of signaling peptides
Xu Wang, Jens Pfannstiel, Annick Stintzi and Andreas Schaller
8 Detection of apoplastic protease inhibitors using convolution activity based proteome profiling
Andrea Passarge, Gunther Doehlemann, Johana C. Misas Villamil
9 Cysteine reactivity profiling to unveil redox regulation in phytopathogens
Kyoko Morimoto, Monika Stegmann, Farnusch Kaschani, Shabaz Mohammed, Renier A. L. van der Hoorn
10 Determination of caspase-like activities in roots by the use of fluorogenic substrates
Katarina Soln, Marina Klemenčič
11 Detection of damage-activated metacaspase activity by Western blot in plants
Simon Stael, Luke P. Miller, Álvaro D. Fernández-Fernández, Frank Van Breusegem
12 Sensitive plant N-terminome profiling with HUNTER
Fatih Demir, Andreas Perrar, Melissa Mantz, Pitter F. Huesgen
13 Profiling sequence specificity of proteolytic activities using proteome-derived peptide libraries
Fatih Demir, Maithreyan Kuppusamy, Andreas Perrar, Pitter F. Huesgen
14 Monitoring Cell Death via Ion leakage and PAM Fluorometry
Nick Dunken, Lisa Mahdi, Rainer E. Häusler, Alga Zuccaro
15 Measuring and perturbing ferroptosis in plants
Ayelen M. Distéfano, Fernanda Marchetti, Eduardo
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