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The Plant Microbiome: Methods and Protocols

The Plant Microbiome: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 2232

Botany & Horticulture

ISBN10: 1071610392
ISBN13: 9781071610398
Publisher: Humana Pr
Published: Nov 8 2020
Pages: 321
Weight: 1.60
Height: 0.87 Width: 7.29 Depth: 10.13
Language: English

This volume provides methods, protocols, and reviews that are useful for new and experienced plant microbiome researchers. Chapters guide readers through the investigation of microbiomes associated with seeds, sampling microbiomes from plant compartments and tissues, culture-based methods, culture-independent metabarcoding methods, methods to obtain DNA and perform metabarcoding, protocols to block PCR amplification from the plant host, qPCR-based methods, editing of specific genes in Bacillus genomes, and Streptomycetes and plant microbial indicators. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Botany & Horticulture