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Plant-Nematode Interactions

Plant-Nematode Interactions

Hardcover

Series: Methods in Molecular Biology, Book 2756

Botany & Horticulture

ISBN10: 1071636375
ISBN13: 9781071636374
Publisher: Humana
Published: Mar 2 2024
Pages: 384
Weight: 2.00
Height: 0.88 Width: 7.00 Depth: 10.00
Language: English
This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their interactions with crop plants. The chapters in this book cover topics such as ways to obtain samples from soil and different plant tissues; rearing nematodes in greenhouse setting; methods used to axenically rear nematodes in vitro; Denaturing Gradient Gel Electrophoresis (DGGE) separation; methods for ROS detection and hydrogen peroxide quantification; methods to elicit plant resistance against nematodes; methods for the detection of Pathogenesis Related (PR) genes that are the executioners of immunity in plant-RKN interactions; and laboratory procedures that test nematodes on their isolation, count, culture, identification, pathogenicity, virulence, and environmental tolerance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Molinari, Sergio

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Botany & Horticulture