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Plant Micrornas: Methods and Protocols

Plant Micrornas: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 592

Medical ReferenceBotany & HorticultureChemistry

ISBN10: 1617796530
ISBN13: 9781617796531
Publisher: Springer Nature
Published: Aug 9 2012
Pages: 261
Weight: 1.08
Height: 0.59 Width: 7.00 Depth: 10.00
Language: English

MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

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Botany & Horticulture