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Plastids: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1829
ISBN13: 9781493986538
Publisher: Springer Nature
Published: Jul 10 2018
Pages: 409
Weight: 2.10
Height: 0.94 Width: 7.00 Depth: 10.00
Language: English
Preface...
Table of Contents...
Contributing Authors...
Part I Plastid Evolution, Plasticity, Structural, and Functional Diversity
1. Primary Endosymbioses: Emergence of the Primary Chloroplast and the Chromatophore, Two Independent Events
Eric Maréchal
2. Complex Endosymbioses I: From Primary to Complex Plastids, Multiple Independent Events
Zoltán Füssy and Miroslav Oborník
3. Complex Endosymbiosis II: The Non-Photosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism
Cyrille Y. Botté and Yoshiki Yamaryo- Botté
4. Diversity and Plasticity of Plastids in Land Plants
Katalin Solymosi, Johanna Lethin, and Henrik Aronsson
5. The Main Functions of Plastids
Norbert Rolland, Imen Bouchnak, Lucas Moyet, Daniel Sali, and Marcel Kuntz
6. Plastid Transient and Stable Interactions with Other Cell Compartments
Stefanie J. Mueller-Schuessele and Morane Michaud
Part II Laboratory Protocols
7. Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy
Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F. Estrozi, Christine Moriscot, Guy Schoehn, Giovanni Finazzi, and Denis Falconet
8. Purification of Chloroplasts and Chloroplast Subfractions: Envelope, Thylakoids, and Stroma - From Spinach, Pea, and Arabidopsis thaliana
Maryse A. Block and Catherine Albrieux
9. Isolation of Inner and Outer Membranes of the Chloroplast Envelope from Spinach and Pea
Maryse A. Block
10. Purification of Non-Green Plastids (Proplastids and Amyloplasts) from Angiosperms, and Isolation of Their Envelope Membranes
Claude Alban and Etienne-Pascal Journet
11. In Vitro Protein Import into Isolated Chloroplasts
Maryse A. Block
12. Analysis of the MTL Super-Complex at Contact Sites between Mitochondria and Plastids
Morgane Michaud
13. Isolation of Plastid-Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum
Alexander Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, and Carolina Rio Bartulos14. Isolating the Plasmodium falciparum Apicoplast using Magnetic Beads
Cyrille Y. Botté, Geoffrey I. McFadden, and Yoshiki Yamaryo-Botté
15. Extraction and Quantification of Lipids from Plant or Algae
Valérie Gros, Josselin Lupette, and Juliette Jouhet16. Quantitative Assessment of the Chloroplast Lipidome
Valérie Gros and Juliette Jouhet
17. Determination of the DNA/RNA-Associated Sub-Proteome from Chloroplasts and Other Plastid Types
Maha Chieb, Monique Liebers, Fabien Chevalier, Silva Lerbs-Mache, Robert Blanvillain, and Thomas Pfannschmidt
18. Mapping Plastid Transcript Population by Circular Reverse Transcription-Polymerase Chain Reaction
Florence Courtois and Livia Merendino
19. Bioinformatic Analysis of Chloroplast Gene Expression and RNA Post-Transcriptional Maturations using RNA Sequencing
Bastien Malbert, Guillen Rigaill, Veronique Brunaud, Claire Lurin, and Etienne Delannoy
20. A Guide to the Chloroplast Transcriptome Analysis using RNA-Seq
Elena J.S. Michel, Amber M. Hotto, Susan R. Strickler, David B. Stern, and Benoît Castandet
21. A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae
Águila Ruiz-Sola and Dimitris Petroutsos
22. Rescue of Deletion Mutants to Isolate Plastid Transformants in Higher Plants
Mohammad El Hajj, Mohd Fahdli Bin Hamdan, Elena Martin Avila, and Anil Day
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