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Plant Proteomics: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1072
BiologyBotany & HorticultureChemistry
ISBN13: 9781493960293
Publisher: Humana
Published: Sep 17 2016
Pages: 786
Weight: 3.04
Height: 1.61 Width: 7.00 Depth: 10.00
Language: English
Part I. Introductory
1. Plant Proteomics Methods and Protocols
Jesús V. JorrÃn Novo
2. From Proteomics to Systems Biology: MAPA, MASS WESTERN, PROMEX and COVAIN as a User-Oriented Platform
Wolfram Weckerth, Stefanie Wienkoop, Volker Egelhofer, and Xiaoliang Sun
3. Plant Proteomics: From Genome Sequencing to Proteome Databases and Repositories
Katsumi Sakata and Setsuko Komatsu
4. How to Use 2D Gel Electrophoresis in Plant Proteomics
Thierry Rabilloud
5. Standarization of Data Processing and Statistical Analysis in a 2-DE-Based Comparative Plant Proteomics Experiment
Luis Valledor, Cristina Romero, and Jesús V. JorrÃn Novo
6. The Expanding Universe of Mass Analyzer Configurations for Biological Analysis
Juan J. Calvete
Part II. Experimental
7. A Protocol for Protein Extraction from Lipid-Rich Plant Tissues Suitable for Electrophoresis
Agnieszka Zienkiewicz, Juan David Rejón, Juan de Dios Alché, MarÃa Isabel RodrÃguez, and Antonio Jesús Castro
8. Making a Protein Extract in Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
Raquel Gonzalez Fernandez, Inmaculada Redondo, and Jesus V. Jorrin Novo.
9. Plant Proteomics Methods to Reach Low-Abundance Proteins
Egisto Boschetti and Pier Giorgio Righetti
10. Combination of 2DE and LC for Plant Proteomics Analysis
Sami Irar, Faiçal Brini, Khaled Masmoudi, and Montserrat Pagès
11. 2DE Analysis of Forest Tree Proteins Using Fluorescent Labels and Multiplexing
Jenny Renaut, Céline Leclercq, and Sébastien Planchon
12. Differential Plant Proteome Analysis by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)
MarÃa J. MartÃnez-Esteso, Juan Casado-Vela, Susana Sellés-Marchart, Mari A. Pedreño, and Roque Bru-MartÃnez
13. Global Quantitative Proteomics Using Spectral Counting: An Inexpensive Experimental and Bioinformatics Workflow for Deep Proteome Coverage
Tiago S. Balbuena, Diogo Ribeiro Demartini, and Jay J. Thelen
14. Combining Chymotrypsin/trypsin Digestion to Identify Hydrophobic Proteins from Oil Bodies
Martina Vermachova, Zita Purkrtova, Jiri Santrucek, Pascale Jolivet, Thierry Chardot, and Milan Kodicek
15. Mass Western for Absolute Quantification of Target Proteins and Considerations About the Instrument of Choice
David Lyon, Wolfram Weckwerth, and Stefanie Wienkoop
16. Selected Reaction Monitoring Mass Spectrometry: A Methodology Overview
Alexander Ebhardt
17. Mass Spectrometry Based Imaging of Metabolites and Proteins
Manuela Peukert, Michael Becker, Andrea Matros, and Hans-Peter Mock
18. Using the Yeast Two-Hybrid System to Identify Protein-Protein Interactions
Eduardo RodrÃguez-Negrete, Eduardo R. Bejarano, and Araceli G. Castillo
19. Modifications of Wheat Germ Cell-Free System for Functional Proteomics of Plant Membrane Proteins
Akira Nozawa and Yuzuru Tozawa
Part III. Biological Systems
20. Arabidopsis Proteomics: A Simple and Standardizable Workflow for Quantitative Proteome Characterization
Anja Rödiger, Birgit Agne, Katja Baerenfaller, and Sacha Baginsky
21. Analysis of Rice Proteins Using SDS-PAGE Shotgun Proteomics
Karlie A. Neilson, Iniga S. George, Samantha J. Emery, Sridevi Muralidharan, and Paul A. Haynes
22. Medicago truncatula Proteomics for Systems Biolog
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