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Plant Endosomes: Methods and Protocols

Plant Endosomes: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 1209

Anatomy & PhysiologyBotany & Horticulture

ISBN10: 1493945866
ISBN13: 9781493945863
Publisher: Humana Pr
Published: Sep 22 2016
Pages: 286
Weight: 1.15
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English

Plant Endosomes: Methods and Protocols explores a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, as well as protein-protein interactions in the endosomal and endomembrane system. The volume continues with coverage of the sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal trafficking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins and the identification of novel endosomal components by chemical genomics and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

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