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Plant Circadian Networks: Methods and Protocols

Plant Circadian Networks: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2398

BiologyBotany & Horticulture

ISBN10: 1071619144
ISBN13: 9781071619148
Publisher: Humana
Published: Oct 23 2022
Pages: 245
Weight: 1.01
Height: 0.55 Width: 7.00 Depth: 10.00
Language: English


This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tagging of clock proteins and cellular imaging, protein level measurements, regulated RNA splicing detection, post-translational modifications, protein-protein interactions, and protein DNA/chromatin interactions. Additional chapters focus on seasonal processes like hypocotyl growth and flowering time control in annuals and trees, and techniques on soybean. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

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Staiger, Dorothee

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Botany & Horticulture