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Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Paperback
BiologyBotany & HorticultureChemistry
ISBN13: 9783319386676
Publisher: Springer Nature
Published: Oct 17 2016
Pages: 38
Weight: 0.18
Height: 0.10 Width: 6.14 Depth: 9.21
Language: English
1 Introduction
1.1 Background
1.2 Methods used to isolate genomic DNA from plant tissues
1.3 Methods for the discovery and characterization of induced and natural nucleotide variation in plant genomes
References
2 Health and Safety Considerations
2.1 Guidelines
2.2 Preparation of a home-made chemical spill kit
References
3 Sample Collection and Storage
3.1 Background
3.2 Materials
3.3 MethodsReferences
4 Low-Cost DNA Extraction
4.1 Materials
4.2 Methods
4.3 Alternative buffers for DNA extraction
References
5 PCR Amplification for Low-Cost Mutation Discovery
5.1 Materials
5.2 Methods
References
6 Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
6.1 Materials6.2 Methods
References
7 Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
7.1 Materials
7.2 Methods
8 Example Data
8.1 Quality of genomic DNA obtained by silica powder-based DNA extraction method
8.2 Quality of genomic DNA obtained by silica powder-based DNA extraction using alternative buffers method
8.3 Example of PCR products using TILLING primers with source genomic DNA from Qiagen kits and low-cost silica method
8.4 Example of low-cost agarose gel based TILLING assays for the discovery of induced point mutations
8.5 Example of enzyme activity recorded from weeds compared to crude celery juice extract
9 Conclusions
1 different editions
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