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Isolation of Plant Organelles and Structures: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1511
ISBN13: 9781493982288
Publisher: Humana
Published: Jun 27 2018
Pages: 338
Weight: 1.35
Height: 0.73 Width: 7.00 Depth: 10.00
Language: English
1. The Isolation of Plant Organelles and Structures in the Post-Genomics Era
A. Harvey Millar and Nicolas L. Taylor
2. Approaches to Characterize Organelle, Compartment, or Structure Purity
Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski
Part I: Isolation of Organelles and Organelle Compartments
3. Isolation of Nuclei and Nucleoli
Alison F. Pendle and Peter J. Shaw
4. Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts
Úrsula Flores-Pérez and Paul Jarvis
5. Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit
Cristina Barsan, Marcel Kuntz, and Jean-Claude Pech
6. Leucoplast Isolation and Subfractionation
William C. Plaxton
7. Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes
Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor
8. Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane
Sigrun Reumann and Piotr Lisik
9. Isolation of Vacuoles and the Tonoplast
Jan Zouhar
10. Isolation of Endoplasmic Reticulum and Its Membrane
G. Eric Schaller
11. Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings
Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, and Joshua L. Heazlewood
12. Isolation of Autolysosomes from Tobacco BY-2 Cells
Chihiro Takatsuka, Yuko Inoue, and Yuji Moriyasu
13. Isolation of Protein Storage Vacuoles and Their Membranes
Tomoo Shimada and Ikuko Hara-Nishimura
Part II: Isolation of Other Structures and Compartments
14. Isolation of the Cell Wall
Hervé Canut, Cécile Albenne, and Elisabeth Jamet
15. Isolation of Plasmodesmata
Christine Faulkner and Emmanuelle M.F. Bayer
16. Isolation of Plasma Membrane and Plasma Membrane Microdomains
Anzu Minami, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura
17. Enrichment of the Plant Cytosolic Fraction
Jeemeng Lao, Andreia M. Smith-Moritz, Jennifer C. Mortimer, and Joshua L. Heazlewood
18. Isolation of Apoplast
Erik Andreasson, Kibrom B. Abreha, and Svante Resj
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