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Biotechnologie und Ingwer
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ISBN10: 6630436407
ISBN13: 9786630436402
Publisher: Verlag Unser Wissen
Published: Aug 23 2026
Pages: 148
Weight: 0.46
Height: 0.34 Width: 6.00 Depth: 9.00
Language: German
ISBN13: 9786630436402
Publisher: Verlag Unser Wissen
Published: Aug 23 2026
Pages: 148
Weight: 0.46
Height: 0.34 Width: 6.00 Depth: 9.00
Language: German
Diese Studie wurde im Zeitraum von 2008 bis 2010 am Lehrstuhl für Pflanzenbiotechnologie des Nationalen Forschungszentrums und am Lehrstuhl für Naturressourcen des Instituts für Afrikaforschung und -studien der Universität Kairo, Ägypten, durchgeführt. Die Studie befasst sich mit der Anwendung pflanzenbiotechnologischer Verfahren an der Ingwerpflanze (Zingiber officinale). Die Ergebnisse zeigten, dass eine In-vitro-Vermehrungsmethode unter Verwendung frischer Keimknospen aus dem Rhizom entwickelt wurde. Explantate, die auf MS-Medium mit 4,5 mg/l BAP kultiviert wurden, wiesen die höchste Triebvermehrungsrate auf. Die Triebe wurden auf einem B5-Medium halber Konzentration mit 1,0 mg/l NAA bewurzelt. Die in vitro gezüchteten Pflänzchen wurden ins Gewächshaus umgepflanzt, wobei ihre Überlebensrate bei 80-100 % lag. Darüber hinaus wurde aus jungen Blättern auf MS-Medium mit 3 mg/l 2,4-D Kallus induziert; zur Regeneration der Triebe wurde der Kallus auf MS-Medium mit 1,0 mg/l BAP subkultiviert. Zudem wurden bei den in vitro induzierten Triebchen nach der Übertragung auf MS-Medium mit 9 mg/l BAP und 60-90 g/l Saccharose bei einer 16-stündigen Photoperiode innerhalb von 10 Wochen Kultivierung Mikrorhizome gebildet. Die DNA-Fingerabdrücke und SDS-PAGE-Marker zeigten eine hohe Ähnlichkeit zwischen den in vitro und in vivo gewonnenen Materialien. Darüber hinaus ergab die HPLC-Analyse einen reinen Einzelpeak von 6-Gingerol.
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