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Interaction entre Rhizobium et les champignons mycorhiziens arbusculaires
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ISBN10: 6209441564
ISBN13: 9786209441561
Publisher: Editions Notre Savoir
Published: Feb 19 2026
Pages: 56
Weight: 0.19
Height: 0.13 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786209441561
Publisher: Editions Notre Savoir
Published: Feb 19 2026
Pages: 56
Weight: 0.19
Height: 0.13 Width: 6.00 Depth: 9.00
Language: French
Phaseolus radiatus et Phaseolus mungo ont été sélectionnés pour la présente étude. Les rhizobiums ont été isolés à partir des deux espèces de Phaseolus sur une gélose à l'extrait de levure et au mannitol (YEMA) avec du rouge Congo. La caractérisation des deux isolats de rhizobiums a été réalisée à partir de cultures âgées de 24 à 48 heures. Des tests biochimiques ont été effectués. Il s'agissait du test de la cétolactase, de la croissance sur gélose au glucose-peptone, de la croissance sur bouillon alcalin de Hofer, de la croissance dans des conditions anaérobies, de la production d'hydrogène sulfuré, de la tolérance aux températures extrêmes et de la résistance à la chaleur, qui se sont tous révélés négatifs. Il a donc été confirmé qu'il s'agissait de Rhizobium. L'effet des champignons AM et du Rhizobium a été étudié à l'aide d'une expérience en bocal Leonard et de cultures en pot ouvert pour les deux espèces de Phaseolus. Des études de culture tissulaire ont été réalisées sur des milieux MS (1962) et BLD (1966) enrichis en régulateurs de croissance. La quantité de protéines, de polyphénols, d'acides nucléiques et de peroxydases a été estimée à partir de cultures doubles et de callosités seules.
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