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Agrobacterium Protocols: Volume II

Agrobacterium Protocols: Volume II

Paperback

Series: Methods in Molecular Biology, Book 344

Botany & Horticulture

ISBN10: 1617378038
ISBN13: 9781617378034
Publisher: Springer Nature
Published: Dec 9 2010
Pages: 512
Weight: 1.49
Height: 1.03 Width: 6.00 Depth: 9.00
Language: English
A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Botany & Horticulture