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Plant Mitochondria: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2363
ISBN13: 9781071616550
Publisher: Humana Pr
Published: Sep 25 2021
Pages: 342
Weight: 1.37
Height: 0.74 Width: 7.00 Depth: 10.00
Language: English
1. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients
Huy Cuong Tran and Olivier Van Aken
2. Cell Type-Specific Isolation of Mitochondria in Arabidopsis
Clément Boussardon and Olivier Keech
3. Isolation of Plant Mitochondria Using Affinity Purification
Franziska Kuhnert and Andreas P.M. Weber
4. Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient
Ian Max M ller and Allan G. Rasmusson
5. Integrity Assessment of Isolated Plant Mitochondria
Allan G. Rasmusson, Mengshu Hao, and Ian Max M ller
6. High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores
Brendan M. O'Leary, Glenda Guek Khim Oh, and A. Harvey Millar
7. Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin
Allan G. Rasmusson, Ian Max M ller, and Susanne Widell
8. Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS)
Xuyen Le, A. Harvey Millar, and Chun Pong Lee
9. Complexome Profiling of Plant Mitochondrial Fractions
Lucie Schröder, Holger Eubel, and Hans-Peter Braun
10. High Throughput BN-PAGE for Mitochondrial Respiratory Complexes
Lilian Vincis Pereira Sanglard and Catherine Colas des Francs-Small
11. Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy
Anqiang Tang, Yimin Wang, and Nicolas L. Taylor
12. Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana
Cristina Ruberti
13. Assessment of Mitochondrial Protein Topology and Membrane Insertion
Kerstin Schäfer, Carina Engstler, Korbinian Dischinger, and Chris Carrie
14. Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis
Malgorzata Kwasniak-Owczarek, Artur Tomal, and Hanna Janska
15. Markers for Mitochondrial ROS Status
Monika Ostaszewska-Bugajska, Anna Podgórska, and Bożena Szal
16. Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling
Jonas Giese, Jürgen Eirich, Frederik Post, Markus Schwarzländer, and Iris Finkemeier
17. Analysis of the Plant Mitochondrial Transcripto
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