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612-822-4611
Plant DNA Fingerprinting and Barcoding: Methods and Protocols

Plant DNA Fingerprinting and Barcoding: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 862

Botany & HorticultureGenetics

ISBN10: 1617796085
ISBN13: 9781617796081
Publisher: Humana
Published: Mar 15 2012
Pages: 202
Weight: 1.27
Height: 0.70 Width: 7.10 Depth: 10.10
Language: English

Molecular cloning and DNA-based analysis have become part of every molecular life science laboratory. The rapid adoption of DNA-based techniques has been facilitated by the introduction of the polymerase chain reaction (PCR), which has made cloning and characterization of DNA quick and relatively simple. PCR is virtually part of every variation of the plethora of approaches used for DNA fingerprinting today. Plant DNA Fingerprinting: Methods and Protocols aims to bring together the different currently available genome-based techniques into one repository. This volume contains detailed protocols for the preparation of plant genomic DNA, fingerprinting of plants for the detection of intra-species variations, the use of DNA barcoding, as well as methods for the bioinformatic analysis of data. Also included are several discussions on the broader issues of genome-based approaches in order to provide a sound understanding of the principles of these methods. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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