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Rozmnażanie mikroklonalne roślin
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ISBN10: 6209382452
ISBN13: 9786209382451
Publisher: Wydawnictwo Nasza Wiedza
Published: Dec 16 2025
Pages: 180
Weight: 0.55
Height: 0.41 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786209382451
Publisher: Wydawnictwo Nasza Wiedza
Published: Dec 16 2025
Pages: 180
Weight: 0.55
Height: 0.41 Width: 6.00 Depth: 9.00
Language: Polish
Niniejszy podręcznik metodyczny stanowi kompleksowy i zorientowany na praktykę przewodnik po mikroklonalnym rozmnażaniu roślin, zaprojektowany specjalnie dla studentów uniwersytetów zajmujących się laboratoryjną biotechnologią roślin. Wprowadza on w podstawowe zasady hodowli tkanek roślinnych, w tym totipotencję komórkową, rolę roślinnych regulatorów wzrostu oraz ścieżki rozwojowe tworzenia kalusa, organogenezy i embriogenezy somatycznej. Podręcznik przedstawia podstawową organizację laboratorium, standardy bezpieczeństwa, funkcje sprzętu i procedury sterylizacji niezbędne do utrzymania aseptycznych warunków. Szczególowa sekcja dotycząca przygotowania pożywek obejmuje sklad skladników odżywczych, formulowanie roztworu podstawowego, regulację pH i autoklawowanie, uzupelnione o cwiczenia dla studentów krok po kroku. Kolejne rozdzialy koncentrują się na wyborze roślin-dawców, typach eksplantatów, technikach sterylizacji powierzchni i zakladaniu kultur. Praktyczne cwiczenia prowadzą studentów przez krytyczne etapy mikrorozmnażania, w tym inokulację, rozmnażanie pędów, ukorzenianie i aklimatyzację zregenerowanych sadzonek.
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