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Propagazione microclonale delle piante
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ISBN10: 6209379893
ISBN13: 9786209379895
Publisher: Edizioni Sapienza
Published: Dec 16 2025
Pages: 180
Weight: 0.55
Height: 0.41 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786209379895
Publisher: Edizioni Sapienza
Published: Dec 16 2025
Pages: 180
Weight: 0.55
Height: 0.41 Width: 6.00 Depth: 9.00
Language: Italian
Questo manuale metodologico fornisce una guida completa e orientata alla pratica della propagazione microclonale delle piante, pensata specificamente per gli studenti universitari impegnati nelle biotecnologie vegetali di laboratorio. Introduce i principi fondamentali della coltura dei tessuti vegetali, tra cui la totipotenza cellulare, il ruolo dei regolatori di crescita delle piante e le vie di sviluppo della formazione del callo, dell'organogenesi e dell'embriogenesi somatica. Il manuale illustra l'organizzazione essenziale del laboratorio, gli standard di sicurezza, le funzioni delle apparecchiature e le procedure di sterilizzazione necessarie per mantenere condizioni asettiche. Una sezione dettagliata sulla preparazione dei terreni di coltura riguarda la composizione dei nutrienti, la formulazione della soluzione madre, la regolazione del pH e l'autoclavaggio, integrata da esercizi per gli studenti passo dopo passo. I capitoli successivi si concentrano sulla selezione delle piante donatrici, sui tipi di espianto, sulle tecniche di sterilizzazione delle superfici e sull'allestimento delle colture. Gli esercizi pratici guidano gli studenti attraverso le fasi critiche della micropropagazione, tra cui l'inoculazione, la moltiplicazione dei germogli, la radicazione e l'acclimatazione delle piantine rigenerate.
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