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Propagação microclonal de plantas
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ISBN10: 620937221X
ISBN13: 9786209372216
Publisher: Edicoes Nosso Conhecimento
Published: Dec 16 2025
Pages: 180
Weight: 0.55
Height: 0.41 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786209372216
Publisher: Edicoes Nosso Conhecimento
Published: Dec 16 2025
Pages: 180
Weight: 0.55
Height: 0.41 Width: 6.00 Depth: 9.00
Language: Portuguese
Este manual metodológico fornece um guia abrangente e orientado para a prática da propagação microclonal de plantas, concebido especificamente para estudantes universitários envolvidos em biotecnologia vegetal baseada em laboratório. Introduz os princÃpios fundamentais da cultura de tecidos vegetais, incluindo a totipotência celular, o papel dos reguladores de crescimento das plantas e as vias de desenvolvimento da formação de calos, organogénese e embriogénese somática. O manual descreve a organização essencial do laboratório, as normas de segurança, as funções do equipamento e os procedimentos de esterilização necessários para manter as condições assépticas. Uma secção detalhada sobre a preparação dos meios de cultura abrange a composição dos nutrientes, a formulação da solução de reserva, o ajuste do pH e a autoclavagem, complementada por exercÃcios passo a passo para os alunos. Os capÃtulos seguintes focam a seleção de plantas dadoras, tipos de explantes, técnicas de esterilização de superfÃcies e o estabelecimento de culturas. ExercÃcios práticos guiam os estudantes através de etapas crÃticas da micropropagação, incluindo inoculação, multiplicação de rebentos, enraizamento e aclimatação de plântulas regeneradas.
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