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Characterizing Osteoblast Mineralization Using Ftir
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ISBN10: 3639253035
ISBN13: 9783639253030
Publisher: Blues Kids Of Amer
Published: May 20 2010
Pages: 116
Weight: 0.40
Height: 0.28 Width: 6.00 Depth: 9.00
Language: English
ISBN13: 9783639253030
Publisher: Blues Kids Of Amer
Published: May 20 2010
Pages: 116
Weight: 0.40
Height: 0.28 Width: 6.00 Depth: 9.00
Language: English
The ability of a cholesterol-reducing drug, simvastatin, to stimulate the formation of hydroxyapatite in an osteoblast-like cell line, was studied using Fourier transform infrared spectroscopy. Hydroxyapatite was detected using both Fourier transform infrared spectroscopy and von Kossa staining techniques a week earlier when cells were cultured in growth media supplemented with simvastatin in a tissue culture polystyrene system. Analysis of infrared spectra using area integration and curve-fitting techniques was performed to quantify the hydroxyapatite content in cells relative to the biological matrix. Infrared spectroscopy was used to quantify the mineralization process. Cells grown in a rotating bioreactor mineralized more rapidly than cells grown in tissue culture polystyrene systems.
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