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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Use of Real-Time Rt-PCR

Use of Real-Time Rt-PCR

Vijayalakshmi, N.

Paperback

Biology

ISBN10: 6208430410
ISBN13: 9786208430412
Publisher: LAP Lambert Academic Publishing
Published: Mar 17 2025
Pages: 64
Weight: 0.22
Height: 0.15 Width: 6.00 Depth: 9.00
Language: English
Foot-and Mouth Disease virus (FMDV) is diagnosed by virus isolation test combined with antigen ELISA and recently one step RT-PCR. Use of fluorogenic RT-PCR is getting more and more importance for rapid detection of FMDV from clinical samples that cannot be processed by regular virus isolation tests and antigen ELISA. Quantitative real-time RT-PCR (qRT-PCR) combines Reverse Transcription with PCR amplification and detection of positive amplification using fluorescent probes like TaqMan (R) probe. In the present study, a TaqMan(R) probe based qRT-PCR was standardized and used for detection of FMDV genome from clinical samples including nasal secretions, plasma and oesophago-pharyngeal fluids (probang samples). The initial standardization was made using different serial dilutions of a FMDV isolate OTNN 24/84 (106.8 TCID50) and 102 virus could be identified using the TaqMan(R) qRT-PCR. RNA standards were synthesized in vitro from a plasmid containing 110 base pair of 3D region of a FMDV type O isolate.

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