Sign-In
Cart
Clonage TA
Paperback
Please select the version of book you would like to purchase.
ISBN10: 6208873908
ISBN13: 9786208873905
Publisher: Editions Notre Savoir
Published: Jul 11 2025
Pages: 60
Weight: 0.21
Height: 0.14 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786208873905
Publisher: Editions Notre Savoir
Published: Jul 11 2025
Pages: 60
Weight: 0.21
Height: 0.14 Width: 6.00 Depth: 9.00
Language: French
Nous avons exploité la technique de clonage TA d'un gène responsable de la résistance aux antibiotiques, à savoir la résistance à la kanamycine, en suivant les étapes de base du processus de clonage. Contexte de l'invention Une condition essentielle pour un génie génétique efficace des bactéries et autres cellules propagées dans des cultures cellulaires est la capacité de sélectionner les cellules présentant une altération génotypique spécifique. La stratégie de sélection la plus courante dans la technologie de l'ADN recombinant consiste à inclure un marqueur de sélection dans le vecteur de clonage ou le plasmide. Un marqueur de sélection peut être un gène cloné ou une séquence d'ADN, qui permet de séparer les cellules hôtes contenant le marqueur de sélection de celles qui ne le contiennent pas. Le marqueur de sélection, associé à un milieu de sélection approprié, maintient le vecteur de clonage dans les cellules. Sinon, étant donné que la réplication des plasmides est une charge énergétique pour l'hôte bactérien, dans une culture en croissance, les bactéries qui ont perdu le plasmide auraient un avantage de croissance par rapport aux cellules contenant le plasmide.
Also from
Vijayalakshmi, N.
Also in
Biology
The Age of Living Machines: How Biology Will Build the Next Technology Revolution
Hockfield, Susan
Paperback
$17.05
Entangled Life: How Fungi Make Our Worlds, Change Our Minds & Shape Our Futures
Sheldrake, Merlin
Paperback
$18.00
Hidden Creatures: Luscious Leeches, Bashful Botflies, and the Wondrous, History-Shaping World of Parasites
Martins, Dino
Hardcover
$27.00
History by a Hair: Thomas Jefferson, Sally Hemings & Solving a 200-Year-Old Mystery
Kurin, Richard
Hardcover
$31.50
Beyond Inheritance: Our Ever-Mutating Cells and a New Understanding of Health
Khamsi, Roxanne
Hardcover
$28.50
The World Beneath Our Feet: The Hidden Life of Soil and Why It Matters to Us All
Ashwood, Frank
Hardcover
$27.00
The Biology of Trauma: How the Body Holds Fear, Pain, and Overwhelm, and How to Heal It
Apigian, Aimie
Hardcover
$26.96
Togetherness: Symbiosis and the Hidden Story of Life's Greatest Collaborations
Hooper, Rowan
Hardcover
$31.50
Go Forth and Forage: A Guide to Foraging Over 50 of the Most Common Edible & Medicinal North American Mushrooms
Johnson, Whitney
Paperback
$23.74
The Power of Life: The Invention of Biology and the Revolutionary Science of Jean-Baptiste Lamarck
Riskin, Jessica
Hardcover
$30.40
Ernst Haeckel's Art Forms in Nature: A Visual Masterpiece of the Natural World
Haeckel, Ernst
Hardcover
$48.74
Fungi Decoded: Adventures in the Mycosphere--Encounters with Thirty Fungi
Bunyard, Britt A.
Paperback
$18.99
Forbidden Facts: Government Deceit & Suppression about Brain Damage from Childhood Vaccines
de Becker, Gavin
Hardcover
$31.34
The Whole Truth: The Hidden Agendas Behind Decades of Health Misinformation--And What to Do about It
Campbell, T. Colin
Campbell, Nelson
Hardcover
$26.55
Princeton Review AP Biology Premium Prep, 29th Edition: 6 Practice Tests + Digital Practice Online + Content Review
The Princeton Review
Paperback
$26.59
Cells at Work! Picture Book 1: The Great Bacteria vs. White Blood Cell Battle!
Makimura, Kumi
Hardcover
$13.29
Night Magic: Adventures Among Glowworms, Moon Gardens, and Other Marvels of the Dark
Henion, Leigh Ann
Hardcover
$28.50
Food Intelligence: The Science of How Food Both Nourishes and Harms Us
Belluz, Julia
Hall, Kevin, PhD
Hardcover
$28.50
Peterson Field Guide to Mushrooms of North America, Second Edition
McKnight, Karl B.
Rohrer, Joseph R.
McKnight Ward, Kirsten
Paperback
$24.69
National Geographic the Photo Ark: One Man's Quest to Document the World's Animals
Sartore, Joel
Chadwick, Douglas H.
Hardcover
From $19.99
Bioinspired: How Nature Made Us Modern, Drives Today's Technological Innovation, and Inspires a Future Worth Inventing
Stier, Samuel Cord
Hardcover
$33.25
The Big One: How We Must Prepare for Future Deadly Pandemics
Osterholm, Michael T.
Olshaker, Mark
Hardcover
$27.00
A Year with the Seals: Unlocking the Secrets of the Sea's Most Charismatic and Controversial Creatures
Morris, Alix
Hardcover
$28.50
All That Remains: A Renowned Forensic Scientist on Death, Mortality, and Solving Crimes
Black, Sue
Paperback
$18.04
Psilocybin: Magic Mushroom Grower's Guide: A Handbook for Psilocybin Enthusiasts
Oeric, O. N.
Oss, O. T.
Paperback
$18.00
VAX Facts: What to Consider Before Vaccinating at All Ages & Stages of Life
Thomas, Paul
Hoover, Deedee
Paperback
$25.60
The Gut-Brain Paradox: Improve Your Mood, Clear Brain Fog, and Reverse Disease by Healing Your Microbiome
Gundry MD, Steven R.
Hardcover
$30.40
The Aesthetic Revolution: Embracing Vanity and the Future of Fitness to Unlock Your Healthiest Self
Israetel, Mike
Hardcover
$28.50
True Hallucinations: Being an Account of the Author's Extraordinary Adventures in the Devil's Paradis
McKenna, Terence
Paperback
$17.09
Psilocybin Mushrooms: A Comprehensive Guide to Growing and Using Psychedelic Mushrooms
Madden, Michael
Paperback
$14.24
Fungi of Temperate Europe: Keys to the Basidiomycotes
Petersen, Jens H.
Frøslev, Tobias G.
Læssøe, Thomas
Hardcover
$90.25
Mushrooms of the Redwood Coast: A Comprehensive Guide to the Fungi of Coastal Northern California
Siegel, Noah
Schwarz, Christian
Paperback
$33.25
Entangled Life: How Fungi Make Our Worlds, Change Our Minds & Shape Our Futures
Sheldrake, Merlin
Hardcover
$28.50
The Little Book of Mushrooms: An Illustrated Guide to the Extraordinary Power of Mushrooms
Dorr, Alex
Hardcover
From $11.99
The Life Machines: How Taking Care of Your Mitochondria Can Transform Your Health
Mochly-Rosen, Daria
Rosen, Emanuel
Hardcover
$27.55
Mushrooming: An Illustrated Guide to the Fantastic, Delicious, Deadly, and Strange World of Fungi
Borsato, Diane
Hardcover
$23.70
The Contagion Myth: Why Viruses (Including Coronavirus) Are Not the Cause of Disease
Cowan, Thomas S.
Fallon Morell, Sally
Hardcover
$31.34
From These Lands: Sharing Our Natural and Cultural Heritage
National Museum of Natural History
Hardcover
$18.95
Mushrooms of Cascadia, Second Edition: An Illustrated Key to the Fungi of the Pacific Northwest
Beug, Michael
Paperback
$26.60
Pull: How Gravity Shapes Your Body, Steadies the Mind, and Guides Our Health
Spiegel, Brennan
Hardcover
$28.50
Human Errors: A Panorama of Our Glitches, from Pointless Bones to Broken Genes
Lents, Nathan H.
Paperback
$17.09
The Ocean's Menagerie: How Earth's Strangest Creatures Reshape the Rules of Life
Harvell, Drew
Hardcover
$28.80
How to Win Friends and Influence Fungi: Collected Quirks of Science, Tech, Engineering, and Math from Nerd Nite
Balakrishnan, Chris
Wasowski, Matt
Paperback
$19.00
The Art and Science of Foodpairing: 10,000 Flavour Matches That Will Transform the Way You Eat
Lahousse, Bernard
Langenbick, Johan
Coucquyt, Peter
Hardcover
$47.45
The Mycocultural Revolution: Transforming Our World with Mushrooms, Lichens, and Other Fungi
McCoy, Peter
Hardcover
$17.05
Field Notes from a Fungi Forager: An Illustrated Journey Through the World of Pacific Northwest Mushrooms
Rodriguez, Ashley
Hardcover
$21.80
The Pale Blue Data Point: An Earth-Based Perspective on the Search for Alien Life
Willis, Jon
Hardcover
$24.70
National Audubon Society Wildflowers of North America: The Complete Identification Reference to Wildflowers--With Full-Color Photographs; Updated Rang
National Audubon Society
Hardcover
$37.95
Gut Check: Unleash the Power of Your Microbiome to Reverse Disease and Transform Your Mental, Physical, and Emotional Health
Gundry MD, Steven R.
Hardcover
$30.40
Field Guide to Wild Mushrooms of Pennsylvania and the Mid-Atlantic: Revised and Expanded Edition
Russell, Bill
Paperback
$23.70
Life in Sync: The Science of Internal Clocks and How We're Disrupting Them
Gander, Philippa
Hardcover
$21.80
The Serengeti Rules: The Quest to Discover How Life Works and Why It Matters
Carroll, Sean B.
Paperback
$18.00
Superconvergence: How the Genetics, Biotech, and AI Revolutions Will Transform Our Lives, Work, and World
Metzl, Jamie
Paperback
$20.89
The Hidden Half of Nature: The Microbial Roots of Life and Health
Biklé, Anne
Montgomery, David R.
Paperback
From $10.99
The Double Helix: A Personal Account of the Discovery of the Structure of DNA
Watson, James D.
Paperback
$18.99
Sea Wonders: The Octopus, the Cuttlefish, and the Squid
Colombo, Marco
Tomasinelli, Francesco
Hardcover
$18.95