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Detecção de anticorpos contra a ascarÃase
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ISBN10: 6209810306
ISBN13: 9786209810305
Publisher: Edicoes Nosso Conhecimento
Published: Apr 10 2026
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786209810305
Publisher: Edicoes Nosso Conhecimento
Published: Apr 10 2026
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Portuguese
A ascarÃase, uma infeção por geo-helmintos em seres humanos causada pelo Ascaris lumbricoides e, ocasionalmente, pelo A. suum, é reconhecida como uma importante infeção parasitária entre as crianças em todo o mundo. No Sri Lanka, foram relatados muitos casos entre pessoas em áreas densamente povoadas em torno de Kalaniya e nas propriedades rurais do interior do paÃs. O diagnóstico da ascarÃase baseia-se atualmente na deteção de ovos de Ascaris nas fezes. Este estudo descreve a extração do antÃgeno larval de Ascaris lumbricoides e o desenvolvimento de um ELISA baseado neste antÃgeno larval para melhorar o diagnóstico serológico da ascarÃase humana. O antÃgeno larval de Ascaris foi extraÃdo a partir de ovos de A. lumbricoides desenvolvidos. A análise do antÃgeno larval foi realizada por SDS-PAGE, seguida de Western Blotting. Foi realizada a determinação da reação cruzada do anticorpo IgG contra Toxocara canis com o antÃgeno larval de Ascaris lumbricoides para avaliar a especificidade do antÃgeno extraÃdo. Os resultados revelaram uma reação cruzada entre os antÃgenos larvais de Ascaris e o soro anti-Toxocara canis. O antÃgeno larval completo não pode ser utilizado para o ELISA, uma vez que conduz a falsos positivos, devido à reação cruzada com outras espécies de ascaróides.
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