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Rilevazione degli anticorpi contro l'ascariasi
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ISBN10: 6209805183
ISBN13: 9786209805189
Publisher: Edizioni Sapienza
Published: Apr 10 2026
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786209805189
Publisher: Edizioni Sapienza
Published: Apr 10 2026
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Italian
L'ascariasi, un'infezione da geoelminti nell'uomo causata dall'Ascaris lumbricoides e occasionalmente dall'A. suum, è riconosciuta come un'importante infezione parassitaria tra i bambini in tutto il mondo. In Sri Lanka, sono stati segnalati molti casi tra le persone che vivono nelle aree densamente popolate intorno a Kalaniya e nelle tenute dell'entroterra. La diagnosi dell'ascariasi si basa attualmente sul rilevamento delle uova di Ascaris nelle feci. Questo studio descrive l'estrazione dell'antigene larvale di Ascaris lumbricoides e lo sviluppo di un test ELISA basato su tale antigene larvale per migliorare la diagnosi sierologica dell'ascariasi umana. L'antigene larvale di Ascaris è stato estratto utilizzando uova di A. lumbricoides sviluppate. L'analisi dell'antigene larvale è stata eseguita mediante SDS-PAGE seguita da Western Blotting. È stata effettuata la determinazione della reazione crociata dell'anticorpo IgG anti-Toxocara canis con l'antigene larvale di Ascaris lumbricoides per valutare la specificità dell'antigene estratto. I risultati hanno rivelato una reazione crociata tra gli antigeni larvali di Ascaris e il siero anti-Toxocara canis. L'antigene larvale intero non può essere utilizzato per l'ELISA poiché porta a falsi positivi, a causa della reazione crociata con altre specie di ascari.
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