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Processo completo di trasferimento genico mediato da Agrobacterium nel tabacco
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ISBN10: 6208944546
ISBN13: 9786208944544
Publisher: Edizioni Sapienza
Published: Jun 17 2025
Pages: 72
Weight: 0.24
Height: 0.17 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786208944544
Publisher: Edizioni Sapienza
Published: Jun 17 2025
Pages: 72
Weight: 0.24
Height: 0.17 Width: 6.00 Depth: 9.00
Language: Italian
L'Agrobacterium tumefaciens è una specie straordinaria di batteri che vive nel suolo e ha la capacità di infettare le cellule vegetali con un frammento del proprio DNA. La trasformazione delle piante mediata dall'A. tumefaciens è diventata il metodo più utilizzato per l'introduzione di geni estranei nelle cellule vegetali e la successiva rigenerazione di piante transgeniche. Il tabacco è una pianta annuale alta e frondosa appartenente alla famiglia delle Solanaceae. Tutte le parti della pianta, ad eccezione dei semi, contengono nicotina, ma la concentrazione è correlata a diversi fattori quali la specie, il tipo di terreno, la coltivazione o le condizioni climatiche. Le annexine sono proteine idrofile solubili in grado di legarsi alla membrana caricata negativamente. Dai risultati della presente ricerca, si è concluso che la trasformazione mediata da A. tumefaciens nel tabacco è un metodo efficace per il trasferimento del gene dell'annexina. Le metodologie possono essere utilizzate anche per la produzione commerciale di tabacco trasformato con tolleranza al sale e alla siccità . Indubbiamente, lo sviluppo di procedure di trasformazione basate sul trasferimento genico mediato da A. tumefaciens per nuove specie di importanza economica è consigliabile e i risultati ottenuti negli ultimi anni evidenziano un futuro promettente.
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