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Processus complet de transfert de gènes médié par Agrobacterium dans le tabac
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ISBN10: 620894452X
ISBN13: 9786208944520
Publisher: Editions Notre Savoir
Published: Jun 17 2025
Pages: 76
Weight: 0.25
Height: 0.18 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786208944520
Publisher: Editions Notre Savoir
Published: Jun 17 2025
Pages: 76
Weight: 0.25
Height: 0.18 Width: 6.00 Depth: 9.00
Language: French
Agrobacterium tumefaciens est une espèce remarquable de bactéries vivant dans le sol qui a la capacité d'infecter les cellules végétales avec un fragment de son ADN. La transformation des plantes médiée par A. tumefaciens est devenue la méthode la plus utilisée pour l'introduction de gènes étrangers dans les cellules végétales et la régénération ultérieure de plantes transgéniques. Le tabac est une plante annuelle à feuilles hautes appartenant à la famille des Solanaceae. Toutes les parties de la plante, à l'exception des graines, contiennent de la nicotine, mais sa concentration dépend de différents facteurs tels que l'espèce, le type de sol, la culture ou les conditions météorologiques. Les annexines sont des protéines solubles et hydrophiles capables de se lier à la membrane chargée négativement. Les résultats de la présente recherche ont permis de conclure que la transformation médiée par A. tumefaciens dans le tabac était une méthode efficace pour le transfert du gène de l'annexine. Ces méthodologies peuvent également être utilisées pour la production commerciale de tabac transformé tolérant au sel et à la sécheresse. Il ne fait aucun doute que le développement de procédures de transformation basées sur le transfert de gènes médié par A. tumefaciens pour de nouvelles espèces importantes sur le plan économique est souhaitable, et les résultats obtenus ces dernières années laissent entrevoir un avenir prometteur.
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