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Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 988
Medical ReferenceTechnology & EngineeringChemistry
ISBN13: 9781493962662
Publisher: Humana
Published: Aug 23 2016
Pages: 355
Weight: 1.42
Height: 0.77 Width: 7.00 Depth: 10.00
Language: English
Part I: Glyco-Engineering of Therapeutic Proteins
1. Engineering of Therapeutic and Diagnostic O-Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells
Linda Lindberg, Jining Liu, and Jan Holgersson
2. Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells
Nassimal El Maï, Sandrine Donadio-Andréi, Chloé Iss, Valérie Calabro, and Catherine Ronin
3. Glycoengineered Pichia-Based Expression of Monoclonal Antibodies
Dongxing Zha
4. N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae
Christelle Arico, Christine Bonnet, and Christophe Javaud
5. Engineering the Baculovirus Genome to Produce Galactosylated Antibodies in Lepidopteran Cells
Sylvie Juliant, Marylêne Lévêque, Pierre Cérutti, Annick Ozil, Sylvie Choblet, Marie-Luce Violet, Marie-Christine Slomianny, Anne Harduin-Lepers, and Martine Cérutti
Part II: Glycoanalytics
6. Nano LC Chips MS/MS for the Characterization of N-Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody
Elsa Wagner-Rousset, Christine Schaeffer-Reiss, Audrey Bednarczyk, Nathalie Corvaïa, Alain Van Dorsselaer, and Alain Beck
7. Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry
Marie-Claire Janin-Bussat, Laure Tonini, Céline Huillet, Olivier Colas, Christine Klinguer-Hamour, Nathalie Corvaïa, and Alain Beck
8. Therapeutic Antibody Glycosylation Analysis: A Contract Research Organization Perspective in the Frame of Batch Release or Comparability Support
Arnaud Delobel, Fabrice Cantais, Anicet Catrain, Erell Dereux, and Géry Van Vyncht
9. Mass Spectrometric Analysis of O-Linked Oligosaccharides from Various Recombinant Expression Systems
Diarmuid T. Kenny, Stefan Gaunit, Catherine A. Hayes, Anki Gustafsson, Magnus Sjöblom, Jan Holgersson, and Niclas G. Karlsson
10. Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC
T. Shantha Raju
11. Application of Capillary Electrophoresis in Glycoprotein Analysis
Richard R. Rustandi, Carrie Anderson, and Melissa Hamm
12. Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology
Grace Chen, Sha Ha, and Richard R. Rustandi
13. Hydrophobic Interaction Chromatography to Analyze Glycoproteins
Richard R. Rustandi
14. Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7
Ludovic Landemarre and Eric Duverger
15. Analysis of Monoclonal Antibodies by Sedimentation Velocity Analytical Ultracentrifugation
W. Blaine Stine, Jr.
Part III: Glycoprotein Complexes Characterization
16. Noncovalent Mass Spectrometry for the Characterization of Antibody/Antigen Complexes
Cédric Atmanene, Elsa Wagner-Rousset, Nathalie Corvaïa, Alain Van Dorsselaer, Alain Beck, and Sarah Sanglier-Cianferani
17. Conformational Analysis of Recombinant Monoclonal Antibodies with Hydrogen/Deuterium Exchange Mass Spectrometry
Damian Houde and John R. Engen
18. Epitope Mapping of Antibodies by Mass Spectroscopy: A Case Study
Victor H. Obungu, Valentina Gelfanova, and Lihua Huang
Part IV: PK/PD Assays for Therapeutic Antibodies
19. Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Des
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