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Isolement et caractérisation de bactéries productrices de lipase
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ISBN10: 6630449533
ISBN13: 9786630449532
Publisher: Editions Notre Savoir
Pages: 80
Weight: 0.26
Height: 0.19 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786630449532
Publisher: Editions Notre Savoir
Pages: 80
Weight: 0.26
Height: 0.19 Width: 6.00 Depth: 9.00
Language: French
La souche bactérienne sélectionnée pour sa production de lipase a été identifiée, sur la base de ses caractéristiques morphologiques, culturales, physiologiques et biochimiques ainsi que d'un arbre phylogénétique établi à l'aide de la méthode Neighbour Joining, comme une nouvelle espèce bactérienne: Staphylococcus argenteus. Cette identification a été confirmée et précisée jusqu'au niveau de l'espèce par IMTECH, à Chandigarh, et l'organisme a été désigné sous le nom de Staphylococcus argenteus souche MG2 MTCC 12820. La séquence nucléotidique de l'ADN ribosomique 16S de l'isolat Staphylococcus argenteus MG2 déterminée dans cette étude a été déposée dans la base de données GeneBank (NCBI, États-Unis) sous le numéro d'accès KY082046. L'organisme Staphylococcus argenteus MG2 a présenté une croissance optimale à un pH de 9,5, ce qui témoigne de sa tolérance aux milieux alcalins. La température optimale de croissance était de 37 C et il était capable de tolérer des concentrations allant jusqu'à 7,5 % de sel et 5 % de saccharose. Des expériences d'optimisation des milieux de culture ont révélé que la source de carbone optimale était le lactose et que la source d'azote optimale était l'extrait de levure. L'organisme Staphylococcus argenteus MG2 s'est révélé être un producteur potentiel de lipase et présente diverses autres applications industrielles.
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