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Izolacja i charakterystyka bakterii wytwarzających lipazę
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ISBN10: 6630354826
ISBN13: 9786630354829
Publisher: Wydawnictwo Nasza Wiedza
Published: Aug 26 2026
Pages: 76
Weight: 0.25
Height: 0.18 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786630354829
Publisher: Wydawnictwo Nasza Wiedza
Published: Aug 26 2026
Pages: 76
Weight: 0.25
Height: 0.18 Width: 6.00 Depth: 9.00
Language: Polish
Hodowla bakteryjna poddana selekcji pod kątem produkcji lipazy zostala zidentyfikowana na podstawie cech morfologicznych, kulturowych, fizjologicznych i biochemicznych, a także drzewa filogenetycznego sporządzonego metodą sąsiedztwa (Neighbour Joining), jako nowy gatunek bakterii Staphylococcus argenteus. Zostalo to dodatkowo potwierdzone i zidentyfikowane do poziomu gatunku przez IMTECH w Chandigarh oraz oznaczone jako szczep Staphylococcus argenteus MG2 MTCC 12820. Sekwencja nukleotydowa 16S rDNA izolatu Staphylococcus argenteus MG2, określona w niniejszym badaniu, zostala zdeponowana w bazie danych GeneBank (NCBI, USA) pod numerem dostępu KY082046. Organizm Staphylococcus argenteus MG2 wykazywal optymalny wzrost przy pH 9,5, co wskazuje na jego tolerancję na środowisko zasadowe. Optymalna temperatura wzrostu wynosila 37 C, a organizm byl w stanie tolerowac stężenie soli do 7,5% oraz stężenie sacharozy do 5%. Eksperymenty optymalizacyjne pożywek wykazaly, że optymalnym źródlem węgla byla laktoza, a źródlem azotu - ekstrakt drożdżowy. Stwierdzono, że organizm Staphylococcus argenteus MG2 jest potencjalnym producentem lipazy i ma wiele innych zastosowań przemyslowych.
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