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Yeast Metabolic Engineering: Methods and Protocols

Yeast Metabolic Engineering: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1152

Medical ReferenceBiology

ISBN10: 1493905627
ISBN13: 9781493905621
Publisher: Springer Nature
Published: Apr 18 2014
Pages: 316
Weight: 2.16
Height: 0.90 Width: 7.20 Depth: 10.10
Language: English

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

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