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Xenopus Protocols: Post-Genomic Approaches
Hardcover
Series: Methods in Molecular Biology, Book 917
ISBN13: 9781617799914
Publisher: Humana
Published: Sep 6 2012
Pages: 568
Weight: 2.69
Height: 1.43 Width: 7.19 Depth: 10.31
Language: English
Part I: Xenopus Tropicalis
1. Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present and Future
Robert M. Grainger
2. Husbandry of Xenopus tropicalis
Alan Jafkins, Anita Abu-Daya, Anna Noble, Lyle Zimmerman, and Matthew Guille
3. Generating Diploid Embryos from Xenopus tropicalis
Florencia del Viso and Mustafa Khokha
4. Navigating the Xenopus tropicalis Genome
Ira L. Blitz
Part II: Genetics in Xenopus
5. Genetic Analysis of Xenopus tropicalis
Timothy J. Geach, Derek L. Stemple, and Lyle B. Zimmerman
6. Forward Genetic Screens in Xenopus using Transposon-mediated Insertional Mutagenesis
Donald A. Yergeau, Clair M. Kelley, Haiqing Zhu, Emin Kuliyev and Paul E. Mead
7. Targeted Gene Disruption with Engineered Zinc-finger Nucleases (ZFNs)
John J. Young and Richard M. Harland
8. Reverse Genetic Studies using Antisense Morpholino Oligonucleotides
Yanan Zhao, Shoko Ishibashi and Enrique Amaya
9. Chemical Genetics and Drug Discovery in Xenopus
Matthew L. Tomlinson, Adam Hendry, and Grant N. Wheeler
10. Maternal mRNA Knock-down Studies: Antisense Experiments using the Host-transfer Technique in Xenopus laevis and Xenopus tropicalis
David J. Olson, Alissa M. Hulstrand and Douglas W. Houston
Part II: Xenopus Trangenesis
11. Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI)
Shoko Ishibashi, Kristen L. Kroll and Enrique Amaya
12. A Simple Method of Transgenesis using I-SceI meganuclease in Xenopus
Shoko Ishibashi, Nick R. Love and Enrique Amaya
13. Using FC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos
You E. Li, Bryan G. Allen and Daniel L. Weeks
14. Xenopus Transgenics: Methods using Transposons
Clair M. Kelley, Donald A. Yergeau, Haiqing Zhu, Emin Kuliyev and Paul E. Mead
15. Comparative Genomics-based Identification and Analysis of Cis-regulatory Elements
Hajime Ogino, Haruki Ochi, Chihiro Uchiyama, Sarah Louie, and Robert M. Grainger
16. Tet-On Binary Systems for Tissue-specific and
Inducible Transgene Expression
Daniel R. Buchholz
Part III: Gene Expression: From Gene to Transcript to Protein
17. Chromatin Immunoprecipitation Analysis of Xenopus Embryos
Robert C. Akkers, Ulrike G. Jacobi and Gert Jan C. Veenstra
18. Transcriptomics Using Next Generation Sequencing Technologies
Dasfne Lee-Liu, Leonardo I. Almonacid, Fernando Faune, Francisco Melo, Juan Larrain
19. Databases of Gene Expression in Xenopus Development
Michael J. Gilchrist, Nicolas Pollet
20. Investigating Alternative RNA Splicing in Xenopus
Agnès Mereau and Serge Hardy
21. Immunoisolation of Protein Complexes from Xenopus
Frank L. Conlon, Yana Miteva, Erin Kaltenbrun, Lauren Waldron, Todd M. Greco, Ileana M. Cristea
22. Complementary Proteomic Analysis of Protein Complexes
Todd M. Greco, Yana Miteva, Frank L. Conlon, Ileana M. Cristea
Part IV: Imaging Xenopus Development
23. Antibody Development and use in Chromogenic and Fluorescent Immunostaining
Eamon Dubaissi, Niki Panagiotaki, Nancy Papalopulu, and Peter D. Vize
24. Multiple fluorescent in situ mRNA Hybridization (FISH) on Whole Mounts and Sections
Robert Lea, Boyan Bonev, Eamon Dubaissi, Peter D. Vize and Nancy Papalopulu
25. Methods to Analyze microRNA Expression and Function During Xenopus Development
Boyan Bonev and Nancy Papalopulu
26. A Bromodeoxyur
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