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Xenopus: Methods and Protocols

Xenopus: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1865

Medical ReferenceOther Animals

ISBN10: 1493987836
ISBN13: 9781493987832
Publisher: Humana Pr
Published: Aug 27 2018
Pages: 302
Weight: 1.82
Height: 0.82 Width: 10.25 Depth: 7.32
Language: English

This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.

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