Sign-In
Cart
Viral Vectors for Gene Therapy: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1937
Medical ReferenceGeneral Psychology
Please select the version of book you would like to purchase.
ISBN10: 1493990640
ISBN13: 9781493990641
Publisher: Humana Pr
Published: Feb 1 2019
Pages: 328
Weight: 1.79
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
ISBN13: 9781493990641
Publisher: Humana Pr
Published: Feb 1 2019
Pages: 328
Weight: 1.79
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
Preface...
Table of Contents...
Contributing Authors...
Matthew J. Benskey, Ivette M. Sandoval, Kathryn Miller, Rhyomi L. Sellnow, Aysegul Gezer, Nathan C. Kuhn, Roslyn Vashon, and Fredric P. Manfredsson Part II Novel Modes of Gene Therapy (Going Beyond Overexpression and Knockdown) 2. Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing
Ivette M. Sandoval, Timothy J. Collier, and Fredric P. Manfredsson 3. Design, Construction, and Application of Transcription Activation-Like Effectors
Peter Deng, Sakereh Carter, and Kyle Fink 4. Practical Considerations for the use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain
Patrick Aldrin-Kirk and Thomas Björklund Part III Viral Vectors 5. AAV Production using Baculovirus Expression Vector System
Quentin Sandro, Karima Relizani, and Rachid Benchaouir 6. Multimodal Production of Adeno-Associated Virus
Ivette M. Sandoval, Nathan M. Kuhn, and Fredric P. Manfredsson 7. Generation of High Titer Pseudotyped Lentiviral Particles
Shuang Hu, Mingjie Li, and Ramesh Akkina 8. A Scalable Lentiviral Vector Production and Purification Method using Mustang Q Chromatography and Tangential Flow Filtration
Stuart Tinch, Kathy Szcur, William Swaney, Lilith Reeves, and Scott R. Witting 9. Current Use of Adenovirus Vectors and their Purification Methods
Ekramy E. Sayedahmed, Rashmi Kumari, and Suresh K. Mittal 10. Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest
Andranik Kahramanian, Toshihiko Kuroda, and Hiroaki Wakimoto 11. Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes
Steven Conrad and Jia Liu Part IV Viral Vector Delivery 12. AAV Mediated Gene Delivery to the Mouse Liver
Sharon C. Cunningham and Ian Alexander 13. Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse
Kevin Isgrig and Wade Chien 14. Gene Transfer to Mouse Kidney In Vivo
C. J. Rocca and S. Cherqui 15. Co-Delivery of a Short-Hairpin RNA and a shRNA-Resistant Replacement Gene with Adeno-Associated Virus: An Allele-Independent Strategy for Autosomal Dominant Retinal Disorders
Michael T. Massengill, Brianna M. Bowman, Alfred S. Lewin, and Cristian J. Ildefonso 16. Localized Intra-Arterial Gene Delivery using AAV
Koji Hosaka, Fredric P. Manfredsson, and Brian Hoh 17. Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors
Francisco Martin, Maria-Tristán-Manzano, Noelia Maldonado, Sabina Sánchez-Hernández, Karim Benabdellah and Marién Cobo 18. Systemic Delivery of Adeno-Associated Viral Vectors in Mice and Dogs
Lakmini P. Wasala, Chady H. Hakim, Yongping Yue, N. Nora Yang, and Donsheng Duan 19. Intrathecal Delivery of AAV Vectors in Cynomolgus Macaques for CNS Gene Therapy and Gene Expression Analysis in Microdissected Motor Neurons
Florel Borel, Eric Adams, and Christian Mueller 20. Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice
Kelsey R. Pfelson, Cristian D. Peterson, Kelley F. Kitto, Lucy Vulchanova, George L. Wilcox, and Carolyn A. Fairbanks 21. Cerebromedullary Cistern Injection of Viral Vectors in Non-Human Primates
Lluis Samaranch, Kousaku Ohno, Waldy San Sebastian, and Krystof Bankiewicz
Peter Deng, Sakereh Carter, and Kyle Fink 4. Practical Considerations for the use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain
Patrick Aldrin-Kirk and Thomas Björklund Part III Viral Vectors 5. AAV Production using Baculovirus Expression Vector System
Quentin Sandro, Karima Relizani, and Rachid Benchaouir 6. Multimodal Production of Adeno-Associated Virus
Ivette M. Sandoval, Nathan M. Kuhn, and Fredric P. Manfredsson 7. Generation of High Titer Pseudotyped Lentiviral Particles
Shuang Hu, Mingjie Li, and Ramesh Akkina 8. A Scalable Lentiviral Vector Production and Purification Method using Mustang Q Chromatography and Tangential Flow Filtration
Stuart Tinch, Kathy Szcur, William Swaney, Lilith Reeves, and Scott R. Witting 9. Current Use of Adenovirus Vectors and their Purification Methods
Ekramy E. Sayedahmed, Rashmi Kumari, and Suresh K. Mittal 10. Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest
Andranik Kahramanian, Toshihiko Kuroda, and Hiroaki Wakimoto 11. Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes
Steven Conrad and Jia Liu Part IV Viral Vector Delivery 12. AAV Mediated Gene Delivery to the Mouse Liver
Sharon C. Cunningham and Ian Alexander 13. Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse
Kevin Isgrig and Wade Chien 14. Gene Transfer to Mouse Kidney In Vivo
C. J. Rocca and S. Cherqui 15. Co-Delivery of a Short-Hairpin RNA and a shRNA-Resistant Replacement Gene with Adeno-Associated Virus: An Allele-Independent Strategy for Autosomal Dominant Retinal Disorders
Michael T. Massengill, Brianna M. Bowman, Alfred S. Lewin, and Cristian J. Ildefonso 16. Localized Intra-Arterial Gene Delivery using AAV
Koji Hosaka, Fredric P. Manfredsson, and Brian Hoh 17. Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors
Francisco Martin, Maria-Tristán-Manzano, Noelia Maldonado, Sabina Sánchez-Hernández, Karim Benabdellah and Marién Cobo 18. Systemic Delivery of Adeno-Associated Viral Vectors in Mice and Dogs
Lakmini P. Wasala, Chady H. Hakim, Yongping Yue, N. Nora Yang, and Donsheng Duan 19. Intrathecal Delivery of AAV Vectors in Cynomolgus Macaques for CNS Gene Therapy and Gene Expression Analysis in Microdissected Motor Neurons
Florel Borel, Eric Adams, and Christian Mueller 20. Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice
Kelsey R. Pfelson, Cristian D. Peterson, Kelley F. Kitto, Lucy Vulchanova, George L. Wilcox, and Carolyn A. Fairbanks 21. Cerebromedullary Cistern Injection of Viral Vectors in Non-Human Primates
Lluis Samaranch, Kousaku Ohno, Waldy San Sebastian, and Krystof Bankiewicz
Also in
Medical Reference
The Spirit Catches You and You Fall Down: A Hmong Child, Her American Doctors, and the Collision of Two Cultures
Fadiman, Anne
Paperback
From $10.99
7 Reasons to Believe in the Afterlife: A Doctor Reviews the Case for Consciousness After Death
Charbonier, Jean Jacques
Paperback
From $8.99
The Immune Mystery: A Doctor's Impassioned Quest to Solve the Puzzle of Autoimmune Disease
Jelstad, Jørgen
Dr Kåss, Anita
Hardcover
$25.60
Holistic Reflexology: Essential Oils and Crystal Massage in Reflex Zone Therapy
Kliegel, Ewald
Paperback
From $10.99
Being Well (Even When You're Sick): Mindfulness Practices for People with Cancer and Other Serious Illnesses
Rosenbaum, Elana
Paperback
From $4.98
The Body Keeps the Score: Brain, Mind, and Body in the Healing of Trauma
Van Der Kolk, Bessel
Paperback
From $12.99
Working The Roots: Over 400 Years of Traditional African American Healing
Lee, Michele Elizabeth
Paperback
$27.55
The Hunger Code: Resetting Your Body's Fat Thermostat in the Age of Ultra-Processed Food
Fung, Jason
Hardcover
$28.45
Integrating a Palliative Approach Workbook 2nd Edition, Revised: Essentials For Personal Support workers
Murray, Katherine
Paperback
$43.50
All Creatures Great and Small: The Warm and Joyful Memoirs of the World's Most Beloved Animal Doctor
Herriot, James
Paperback
From $10.99
Unlearn Your Pain: The Science of Recovering from Chronic Pain, Fatigue, Anxiety, and Depression
Schubiner, Howard, MD
Hardcover
$28.80
Cialis & Men's Sexual Health: Understanding Tadalafil and Erectile Dysfunction
Stilton, John P.
Paperback
$15.19
THE DYSAUTONOMIA PROJECT Second Edition
Thompson, Charles R.
Freeman, Kelly
Goldstein, David S.
Paperback
$31.25
Jesus & Your Mental Health: Linking God's Word and Modern Science to Find Peace about Mental Health
Maxwell Lmft, Rebecca
Paperback
$16.14
You've Been Pooping All Wrong: How to Make Your Bowel Movements a Joy
Pasricha, Trisha, MD MPH
Hardcover
$28.50
Food Is Medicine: Healing Our Bodies, Nourishing Our Minds, and Transforming Our Food System
Mozaffarian, Dariush
Hardcover
$31.34
Anatomy Coloring Book with 450+ Realistic Medical Illustrations with Quizzes for Each
McCann, Stephanie
Wise, Eric
Paperback
$28.49
The 36-Hour Day: A Family Guide to Caring for People with Alzheimer Disease, Other Dementias, and Memory Loss
Rabins, Peter V.
Mace, Nancy L.
Paperback
$23.70
How to Forage for Mushrooms Without Dying: An Absolute Beginner's Guide to Identifying 29 Wild, Edible Mushrooms
Hyman, Frank
Paperback
$15.29
The Mindful Self-Compassion Workbook: A Proven Way to Accept Yourself, Build Inner Strength, and Thrive
Neff, Kristin
Germer, Christopher
Paperback
$21.80
The Invincible Brain: The Clinically Proven Plan to Age-Proof Your Brain and Stay Sharp for Life
Fotuhi, Majid
Hardcover
$30.40
The Breast Advice: All You Need to Know about Breast Health, Screening, and Treatment
Port, Elisa
Hardcover
$28.49
Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (Dsm-5-Tr(r))
American Psychiatric Association
Paperback
$224.40
Period Repair Manual: Natural Treatment for Better Hormones and Better Periods
Briden Nd, Lara
Paperback
$18.95
Living Life to the Fullest with Ehlers-Danlos Syndrome: Guide to Living a Better Quality of Life While Having EDS
Muldowney Pt, Kevin
Muldowney Pt, Kathleen
Hardcover
$69.94
Mayo Clinic Guide to a Healthy, Happy Heart: Simple Steps to Achieving Long-Term Cardiovascular Health
Lerman, Amir
Lara-Breitinger, Kyla M.
Lopez-Jimenez, Francisco
Hardcover
$26.09
Taking Charge of Adult ADHD: Proven Strategies to Succeed at Work, at Home, and in Relationships
Barkley, Russell A.
Paperback
$18.95
Think Less, Sleep More: From Panic & Perfectionism to Stress-Free Sleep
Romiszewski, Stephanie
Hardcover
$28.50
Plant Powered Plus: Activate the Power of Your Gut to Tame Inflammation and Reclaim Your Health
Bulsiewicz, Will
Hardcover
$28.80
The Ultimate Survival Medicine Guide: Emergency Preparedness for Any Disaster
Alton, Amy
Alton, Joseph
Paperback
$18.99
El Cuerpo Lleva La Cuenta: Cerebro, Mente Y Cuerpo En La Superación del Trauma / The Body Keeps the Score
Van Der Kolk, Bessel
Paperback
$17.05
Nolo's Guide to Social Security Disability: Getting & Keeping Your Benefits
Morton III, David A.
Paperback
$37.99
The New Menopause: Navigating Your Path Through Hormonal Change with Purpose, Power, and Facts
Haver, Mary Claire
Hardcover
$26.60
Trauma and Recovery: The Aftermath of Violence---From Domestic Abuse to Political Terror
Herman, Judith Lewis
Paperback
From $12.99
No One's Coming: The Rogue Heroes Our Government Turns to When There's Nowhere Else to Turn
Hazzard, Kevin
Hardcover
$28.50
Cancer Is a Parasite: Kill It with the Safe, Over-The-Counter Antiparasitic Fenbendazole
Supple, William F., Jr.
Hardcover
$31.34
Other Minds: The Octopus, the Sea, and the Deep Origins of Consciousness
Godfrey-Smith, Peter
Paperback
$19.00
Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (Dsm-5-Tr(r))
American Psychiatric Association
Hardcover
$290.40
The Great Shadow: A History of How Sickness Shapes What We Do, Think, Believe, and Buy
Bauer, Susan Wise
Hardcover
$27.00
Medicare 101: From Your Initial Enrollment Period to Parts A, B, C, and D, an Essential Primer on the Government Healthcare Program
Lankford, Kimberly
Hardcover
$16.15
Spiritual Portraits of the Energy Release Points: A Compendium of Acupuncture Point Messages Found Within the 12 Meridians and 8 Extraordinary Vessels
Gervais, Michele Marie
Paperback
$44.99
Why We Get Sick: The Hidden Epidemic at the Root of Most Chronic Disease--And How to Fight It
Bikman, Benjamin
Paperback
From $11.99
Desk Reference to the Diagnostic Criteria from Dsm-5-Tr(r)
American Psychiatric Association
Paperback
$99.00
What Happened to You?: Conversations on Trauma, Resilience, and Healing
Perry, Bruce D., M. D. PH. D.
Winfrey, Oprah
Hardcover
From $11.99
The Boy Who Was Raised as a Dog: And Other Stories from a Child Psychiatrist's Notebook--What Traumatized Children Can Teach Us about Loss, Love, and
Szalavitz, Maia
Perry, Bruce D.
Paperback
$20.89
This Book May Cause Side Effects: The Curious and Dangerous Power of the Nocebo Effect, Placebo's Evil Twin
Pilcher, Helen
Hardcover
$27.00
Stay Off My Kitchen Table: A Heart Surgeon's 4-Week Elimination Diet for Gut Repair, Metabolic Health, and Longevity
Lisec, Joshua
Ovadia, Philip
Paperback
$28.45
God's Hotel: A Doctor, a Hospital, and a Pilgrimage to the Heart of Medicine
Sweet, Victoria
Paperback
$17.10
Harrison's Guide to Inpatient Medicine: Common Internal Medicine Diseases and Workups
Longo, Dan L.
Fauci, Anthony S.
Edwards, Mitchell
Hardcover
$70.00
Medical Apartheid: The Dark History of Medical Experimentation on Black Americans from Colonial Times to the Present
Washington, Harriet A.
Paperback
From $10.99
Destiny of the Republic: A Tale of Madness, Medicine and the Murder of a President
Millard, Candice
Paperback
From $9.99
How to Forage for Medicinal Plants Without Dying: An Absolute Beginner's Guide to Identifying 32 Healing Wild Herbs
Ruiz, Brandon
Paperback
$16.14
Cure Your Fatigue, Second Edition: The Root Cause and How to Fix It on Your Own
Robbins, Morley M.
Paperback
$20.85
The Spirit Catches You and You Fall Down: A Hmong Child, Her American Doctors, and the Collision of Two Cultures
Fadiman, Anne
Paperback
$18.05
Desk Reference to the Diagnostic Criteria from Dsm-5-Tr(r)
American Psychiatric Association
Paperback
$99.00
Fenbendazole & Ivermectin Protocols Handbook: A Definitive Guide on Treatment of Cancer for Patients and Caregivers
Willmott, Sebastian
Paperback
$18.18
Curved Air: A Biography of Sickle Cell Anemia and the Quest to Cure the First Molecular Disease
Davies, Kevin
Hardcover
$26.96
Child Medication Fact Book for Psychiatric Practice, Second Edition
Feder, Joshua D.
Puzantian, Talia
Tien, Elizabeth
Paperback
$80.70
How to Change Your Mind: What the New Science of Psychedelics Teaches Us about Consciousness, Dying, Addiction, Depression, and Transcendence
Pollan, Michael
Paperback
From $11.99
The Cortisol Cure: The Secret to Resetting Your Stress Response, Boosting Energy & Feeling Better Than Ever
Kinney, Erin
Hardcover
$28.50
The Obesity Code: Unlocking the Secrets of Weight Loss (Why Intermittent Fasting Is the Key to Controlling Your Weight)
Fung, Jason
Paperback
From $10.99
Nursing School Entrance Exams Prep: Your All-In-One Guide to the Kaplan and Hesi Exams
Kaplan Nursing
Paperback
$37.99
Good Moms Have Scary Thoughts: A Healing Guide to the Secret Fears of New Mothers
Kleiman, Karen
Hardcover
$18.04
The Doctors' Riot of 1788: Body Snatching, Bloodletting, and Anatomy in America
McPhee, Andy
Hardcover
$26.96
Medical Terminology Made Simple: Easily Learn, Memorize, and Pronounce Medical Terms
Temple, John, MD
Paperback
$14.24
The Lithium Revolution: Optimizing Mood, Memory, and Lifelong Brain Health With Nutritional Lithium
Greenblatt, James
Paperback
$21.84
American Sirens: The Incredible Story of the Black Men Who Became America's First Paramedics
Hazzard, Kevin
Paperback
$18.99
The Other Side of the Straitjacket: A Daughter's Story of Mental Illness and Assisted Dying
Duncan, Alicia
Paperback
$18.95
Indoor Epidemic: 93% Inside Steals Sleep, Focus & Years-The 7% Outdoor Rx Restores Them
La Puma, John
Paperback
$18.99
Menopause: What Your Ob-GYN Wants You to Know
Obstetricians and Gynecologists, American College of
Paperback
$20.89
Hidden Epidemic: Silent Oral Infections Cause Most Heart Attacks and Breast Cancers
Levy, Jd
Paperback
$37.44
Current Medical Diagnosis & Treatment 2027
Rabow, Michael W.
McQuaid, Kenneth R.
Papadakis, Maxine A.
Hardcover
$126.00
Change Your Brain, Change Your Pain: Breaking the Doom Loop to Heal Chronic Physical and Emotional Pain
Amen, Daniel G.
Hardcover
$31.50
Shoulder Pain? The Solution & Prevention: Fifth Edition, Revised & Expanded
Kirsch, John M.
Paperback
$34.93
The Cost of Care: How Four Nurses Put Patients First in a Profit-Driven System
Brown, Theresa
Hardcover
$28.50
Everything Is Tuberculosis: The History and Persistence of Our Deadliest Infection (Large Print Edition)
Green, John
Paperback
$28.50
Gut and Psychology Syndrome: Natural Treatment for Autism, Dyspraxia, A.D.D., Dyslexia, A.D.H.D., Depression, Schizophrenia, 2nd Edition
Campbell-McBride, Natasha, MD
Paperback
$31.30
The Quantum Power of GLP-1 Peptides: Unlocking the Science that Leads to Lasting Weight Loss and Optimal Healthspan
Seeds, William A.
Paperback
$34.20
What Your Doctor Doesn't Know about Fibromyalgia: Why Doctors Can't or Won't Treat Chronic Pain
Meilink, Linda
Rhoades, Patrick
Paperback
$15.15
Clinical Reasoning: Coaching the Struggling Medical Learner
Parsons, Andrew S.
Warburton, Karen M.
Paperback
$78.30
The Flying Death: Poison, Plunder, and the Quest for a Miracle Medicine in the Amazon
O'Hara, Matt
Hardcover
$30.40
Pcos Is My Power: Understanding Pcos, Fixing Your Hormones, and Resetting Your Health
Ruth, Cory, Rdn
Paperback
$21.85
What Happened to You?: Conversations on Trauma, Resilience, and Healing
Winfrey, Oprah
Perry, Bruce D., M. D. PH. D.
Paperback
$17.99
The Cure for Everything: The Epic Struggle for Public Health and a Radical Vision for Human Thriving
Williams, Michelle A.
Hardcover
$30.40
The Unfragile Mind: A Physician's Call for Restoring Hope and Humanity to Mental Health Care
Francis, Gavin
Hardcover
$28.45