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Vegf Signaling: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2475
Medical ReferenceAnatomy & Physiology
ISBN13: 9781071622162
Publisher: Humana
Published: Apr 23 2022
Pages: 353
Weight: 1.88
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
1. Opposing Roles of Vascular Endothelial Growth Factor C in Metastatic Dissemination and Resistance to Radio/Chemotherapy: Discussion of Mechanisms and Therapeutic Strategies
Christopher Montemagno, Frédéric Luciano, and Gilles Pagès
2. Identification of VEGF Isoforms in Mouse, Rat, and Zebrafish Using RT-qPCR
Patricia de Winter
3. Multi-Parameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells
Theresa Connor, Nerida Sleebs, and Zerina Lokmic-Tomkins
4. Absolute Quantification of Plasma Membrane Receptors via Quantitative Flow Cytometry
Yingye Fang, Manasi Malik, Sarah K. England, and P.I. Imoukhuede
5. Measurement of VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo Angiogenesis Functional Assays
Ye Zeng, Xiaoqiang Du, Wenli Jiang, and Yan Qiu
6. SH2-Domain Protein Isolation Using Synthetic Phosphorylated Peptides to Study VEGFR2 Signaling
Chiara Testini
7. Monitoring VEGF-Stimulated Calcium Ion Flux in Endothelial Cells
William R. Critchley, Gareth W.F. Fearnley, Izma Abdul-Zani, Carmen Molina-Paris, Claus Bendtsen, Ian C. Zachary, Michael A. Harrison, and Sreenivasan Ponnambalam
8. Co-Immunoprecipitation Assays
Ian M. Evans and Ketevan Paliashvili
9. Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling
James A.G.E. Taylor, Christopher J. Benwell, and Stephen D. Robinson
10. RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens
Tiziana Annese, Roberto Tamma, and Domenico Ribatti
11. Global MicroRNA Profiling of Vascular Endothelial Cells
Eloi Schmauch, Anna-Liisa Levonen, and Suvi Linna-Kuosmanen
12. Endothelial Cell Tube Formation Assay: An In Vitro Model for Angiogenesis
Mary Kelley, Sara Fierstein, Laura Purkey, and Kathleen DeCicco-Skinner
13. Use of a Thin Layer Assay for Assessing the Angiogenic Potential of Endothelial Cells In Vitro
James A.E. Lane, Ashton Faulkner, Elizabeth J.T. Finding, Eleanor G. Lynam, and Caroline P.D. Wheeler-Jones
14. VEGF-A165 -Induced Endothelial Cells Chemotactic Migration and Invasion Assays
Caroline Pellet-Many
15. Measuring Mitochondrial Calcium Fluxes in Cardiomyocyte upon Mechanical Stretch-Induced Hypertrophy
Daniela Ramaccini, Carlotta Giorgi, and Michelle L. Matter
16. Simultaneous Measurement of Endothelial Cell Proliferation and Cell Cycle Stage Using Flow Cytom
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