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The Mitotic Spindle: Methods and Protocols

The Mitotic Spindle: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1413

Biology

ISBN10: 1493935402
ISBN13: 9781493935406
Publisher: Springer Nature
Published: May 20 2016
Pages: 424
Weight: 2.15
Height: 1.00 Width: 7.00 Depth: 10.00
Language: English

This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.

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