Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
The Crac Channel: Methods and Protocols

The Crac Channel: Methods and Protocols

Penna, Aubin
Constantin, Bruno

Hardcover

Series: Methods in Molecular Biology, Book 1843

BiologyChemistry

ISBN10: 149398702X
ISBN13: 9781493987023
Publisher: Humana Pr
Published: Sep 10 2018
Pages: 204
Weight: 1.32
Height: 0.56 Width: 7.00 Depth: 10.00
Language: English
This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learningmore about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.

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