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The Bacterial Flagellum: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1593
ISBN13: 9781493969265
Publisher: Humana Pr
Published: Apr 8 2017
Pages: 326
Weight: 1.78
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
Preface...
Table of Contents...
Contributors...
Part I Flagellar Type III Protein Export, Assembly and Gene Regulation in Salmonella enterica
1. Fuel of the Bacterial Flagellar Type III Protein Export Apparatus
Tohru Minamino, Miki Kinoshita and Keiichi Namba
2. Interactions of Flagellar Structural Subunits with the Membrane Export Components Machinery
Lewis D.B. Evans, Paul M. Bergen, Owain J. Bryant and Gillian M. Fraser
Marc Erhardt4. Coupling of Flagellar Gene Expression with Assembly in Salmonella enterica
Fabienne F.V. Chevance and Kelly T Hughes
5. Dynamic Measures of Flagellar Gene Expression
Santosh Koirala and Christopher V. Rao
Part II Structure of the Bacterial Flagellum
6. Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella entericaShin-Ichi Aizawa7. Design and Preparation of the Fragment Proteins of the Flagellar Components Suitable for X-Ray Crystal Structure Analysis
Katsumi Imada8. Structural Analysis of the Flagellar Component Proteins in Solution by Small Angle X-Ray Scattering
Lawrence K. Lee
9. Structural Study of the Bacterial Flagellar Basal Body by Electron Cryomicroscopy and Image Analysis
Akihoro Kawamoto and Keiichi Namba
10. Structure of the MotA/B Proton Channel
Akio Kitao and Yasutaka Nishihara
11. Mechanism of Stator Assembly and Incorporation into the Flagellar Motor
Seiji Kojima
Part III Dynamics of the Bacterial Flagellar Motor
12. Rotation Measurements of Tethered Cells
Yuichi Inoue
13. Tracking the Movement of a Single Prokaryotic Cell in Extreme Environmental Conditions
Masayoshi Nishiyama and Yoshiyuki Arai
14. Measurements of the Rotation of the Flagellar Motor by Bead Assay
Taishi Kasai and Yoshiyuki Sowa
15. Measurements of Ion-Motive Force Across the Cell Membrane
Tsai-Shun Lin, Yi-Ren Sun, and Chien-Jung Lo
16. Stoichiometry and Turnover of the Stator and Rotor
Yusuke V. Morimoto and Tohru Minamino
17. Direct Imaging of Intracellular Signaling Molecule Responsible for the Bacterial Chemotaxis
Hajime Fukuoka
18. In Situ Structural Analysis of the Spirochetal Flagellar Motor by Cryo-Electron Tomography
Shiwei Zhu, Zhuan Qin, Juyu Wang, Dustin R. Morado, and Jun Liu
19. Motility of Spirochetes
20. Structure of the Sodium-Driven Flagellar Motor in Marine Vibrio
Yasuhiro Onoue and Michio Homma
21. Chemotactic Behaviors of Vibrio Cholerae Cells
Ikuro Kawagishi and So-ichiro Nishiyama
22. Purification of Fla2 Flagella of Rhodobacter sphaeroides
Javier de la Mora, Laura Camarena, and Georges Dreyfus
23. Dynamics in the Dual Fuel Flagellar Motor of Shewanella oneidensis MR-1
Susanne Brenzinger and Kai M. Thormann
24. Ion Selectivity of the Flagellar Motors Derived from the Alkaliphilic Bacillus and Paenibacillus Species
Yuka Takahashi and Masahiro Ito
25. Measurement of Free-Swimming Motility and Magnetotactic Behavior of Magnetococcus massalia Strain MO-1
Wei-Jia Zhang, Sheng-Da Zhang, and Long-Fei Wu
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