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Th9 Cells: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1585
Please select the version of book you would like to purchase.
ISBN10: 1493968769
ISBN13: 9781493968763
Publisher: Humana Pr
Published: May 5 2017
Pages: 259
Weight: 1.52
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
ISBN13: 9781493968763
Publisher: Humana Pr
Published: May 5 2017
Pages: 259
Weight: 1.52
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
Preface...
Table of Contents...
Contributing Authors..
Ritobrata Goswami 2. IL-9: Function, Sources, and Detection
Wilmer Gerardo Rojas-Zuleta and Elizabeth Sanchez 3. IL-9 Signaling Pathway: An Update
Dijendra Nath Roy and Ritobrata Goswami 4. A Method to In Vitro Differentiate Th9 Cells from Mouse Naïve CD4+ T Cells
Duy Pham 5. T Cell Receptor and Co-Stimulatory Signals for Th9 Generation
Françoise Meylan and Julio Gomez-Rodriguez 6. Polarizing Cytokines for Human Th9 Cell Differentiation
Prabhakar Putheti 7. Determining the Frequencies of Th9 Cells from Whole Blood
Anuradha Rajamanickam and Subash Babu 8. IL-9 Production by Nonconventional T Helper Cells
Silvia C. P. Almeida and Luis Graca 9. Prediction and Validation of Transcription Factors Binding Sites in the IL-9 Locus
William Orent and Wassim Elyaman 10. Flow Cytometric Assessment of STAT Molecules in Th9 Cells
Lucien P. Garo, Vanessa Beynon, and Gopal Murugaiyan 11. Transcription Factors Downstream of IL-4 and TGF- β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
Atsushi Sugimoto, Ryoji Kawakami, and Norihisa Mikami 12. Retroviral Transduction and Reporter Assay: Transcription Factor Co-Operation in Th9 Cell Development
Rukhsana Jabeen 13. Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid-Luminescent DNA-Precipitation Assay
Etienne Humblin, François Ghiringhello, and Frédérique Vegran 14. Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation
Alla Skapenko and Hendrik Schulze-Koops 15. Allergic Inflammation and Atopic Disease: Role of Th9 Cells
Pornpimon Angkasekwinai 16. Characterization of T Helper 9 (Th9) Cells in the Development of EAE and IBD
Sakshi Malik, Valerie Dardalhon, and Amit Awasthi 17. B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer
Alka Dwivedi, Sushant Kumar, and Rahul Purwar 18. Th9 Cells and Parasitic Inflammation: Use of Nippostrongylus and Schistosoma Models
Miguel Enrique Serrano Pino and Paula Licona-Limón 19. Isolation and Purification of Th9 Cells for the Study of Inflammatory Diseases in Research and Clinical Settings
Patricia Keating and James X. Hartmann
Françoise Meylan and Julio Gomez-Rodriguez 6. Polarizing Cytokines for Human Th9 Cell Differentiation
Prabhakar Putheti 7. Determining the Frequencies of Th9 Cells from Whole Blood
Anuradha Rajamanickam and Subash Babu 8. IL-9 Production by Nonconventional T Helper Cells
Silvia C. P. Almeida and Luis Graca 9. Prediction and Validation of Transcription Factors Binding Sites in the IL-9 Locus
William Orent and Wassim Elyaman 10. Flow Cytometric Assessment of STAT Molecules in Th9 Cells
Lucien P. Garo, Vanessa Beynon, and Gopal Murugaiyan 11. Transcription Factors Downstream of IL-4 and TGF- β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
Atsushi Sugimoto, Ryoji Kawakami, and Norihisa Mikami 12. Retroviral Transduction and Reporter Assay: Transcription Factor Co-Operation in Th9 Cell Development
Rukhsana Jabeen 13. Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid-Luminescent DNA-Precipitation Assay
Etienne Humblin, François Ghiringhello, and Frédérique Vegran 14. Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation
Alla Skapenko and Hendrik Schulze-Koops 15. Allergic Inflammation and Atopic Disease: Role of Th9 Cells
Pornpimon Angkasekwinai 16. Characterization of T Helper 9 (Th9) Cells in the Development of EAE and IBD
Sakshi Malik, Valerie Dardalhon, and Amit Awasthi 17. B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer
Alka Dwivedi, Sushant Kumar, and Rahul Purwar 18. Th9 Cells and Parasitic Inflammation: Use of Nippostrongylus and Schistosoma Models
Miguel Enrique Serrano Pino and Paula Licona-Limón 19. Isolation and Purification of Th9 Cells for the Study of Inflammatory Diseases in Research and Clinical Settings
Patricia Keating and James X. Hartmann
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