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Techniques in Free Radical Research: Volume 22
Paperback
Series: Laboratory Techniques in Biochemistry and Molecular Biology, Book 22
Medical ReferenceBiologyChemistry
ISBN13: 9780444813145
Publisher: Elsevier Science
Published: Dec 12 1991
Pages: 290
Weight: 0.67
Height: 0.65 Width: 5.00 Depth: 8.00
Language: English
Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or FOOTPRINTS of radical-mediated damage.
Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.
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