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Trinucleotide Repeat Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1010
ISBN13: 9781627034104
Publisher: Humana
Published: Jun 11 2013
Pages: 296
Weight: 1.60
Height: 0.90 Width: 7.10 Depth: 10.00
Language: English
Part I. Introduction for Trinucleotide Repeat diseases
1. A Brief History of Triplet Repeat Disease
Helen Budworth and Cynthia McMurray
2. Huntington's Disease and Cell Therapies: Past, Present and Future
Chrystalina A. Antoniades and Colin Watts
Part II. Analysis of synaptic plasticity
3. Transcranial Two-Photon Imaging of Synaptic Structures in the Cortex of Awake Head-Restrained Mice
Guang Yang, Feng Pan, Paul C. Chang, Frank Gooden, and Wen-Biao Gan
4. In vivo Imaging of Synapse Plasticity in the Mouse Motor Cortex
Yi Zuo, Xinzhu Yu, Kelly Tennant, and Theresa Jones
Part III. Targeting on ES cells
5. Pluripotent Hybrid Stem Cells from Transgenic Huntington's Disease Monkey
Chuti Laowtammathron and Anthony W.S. Chan
6. Mesenchymal Stem Cells for Trinucleotide Repeat Disorders
Geralyn Annett, Gerhard Bauer, and Jan A. Nolta
Part IV. Targeting on RNA
7. Lentiviral-Mediated Gene Transfer of siRNAs for the Treatment of Huntington's Disease
Karine Cambon and Nicole Déglon
8. Purification of dFMR1-Containing Complexes Using Tandem Affinity Purification
Keita Miyoshi, Akiyo Ogino, Mikiko C. Siomi, and Haruhiko Siomi
9. Combined FISH and Immunofluorescent Staining Methods to Co-localize Proteins and mRNA in Neurons and Brain Tissue
Bin Ma and Naoko Tanese
Part V. Modification of Proteins and DNAs
10. Epigenetic Modifications of the FMR1 Gene
Elisabetta Tabolacci and Giovanni Neri
11. The CGG Repeat and the FMR1 Gene
Renate K. Hukema and Ben A. Oostra
12. Study of Oxidative Damage and Antioxidant Systems in Two Huntington s Disease Rodent Models
Francisca Pérez-Severiano, Sergio Montes-López, Cristian Gerónimo-Olvera, and José Segovia
13. Regulation of Ataxin-1 Phosphorylation and Its Impact on Biology
Sarita Lagalwar and Harry T. Orr
Part VI. Trinucleotide repat expansion and detection
14. Towards Understanding Region-Specificity of Triplet Repeat Diseases: Coupled Immunohistology and Mass Spectrometry Imaging
Virginia Platt, Do Yup Lee, Christie Canaria, Ken Frankel, Susan Bernstein, and Cynthia McMurray
15. Antibodies and Intrabodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant Forms
Ali Khoshnan, Susan Ou, Jan Ko, and Paul H. Patterson
16. Cell Recovery from DM1 Transgenic Mouse Tissue to Study (CTG)n Instability and DM1 Pathogenesis
Judith R. Brouwer, Laurent Foiry, and Geneviève Gourdon
17. Markerless Modification of Trinucleotide Repeat Loci in BACs
Kellie A. Benzow and Michael D. Koob
18. DRPLA: Recent Advances in Research Using Transgenic Mouse Models
Kazushi Suzuki, Toshiya Sato, Mitsunori Yamada, Hitoshi Takahashi, and Shoji Tsuji
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