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Total Internal Reflection Fluorescence (TIRF) and Evanescence Microscopies: Total internal reflection excitation and near field emission
Hardcover
Series: Biophysical Society-Iop
Technology & EngineeringBiology
ISBN13: 9780750333498
Publisher: Institute of Physics Publishing
Published: Sep 13 2022
Pages: 200
Weight: 1.24
Height: 0.50 Width: 7.00 Depth: 10.00
Language: English
This book offers a complete presentation of the physics, math, and experimental setups for both TIRF and related evanescence microscopies. It covers evanescence in both fluorescence excitation or emission. It also discusses, in detail, the theory, setups, and practical biological/biochemical applications for combinations of evanescence microscopies with other optical techniques such as polarization, photobleaching, correlation spectroscopy, scattering, image enhancement, optical force, two-photon, energy transfer, structured illumination, scanning, quenching, and single molecule detection. Physical and qualitative discussions augment the rigorous math, making the book accessible and interesting to a wide range of audiences with backgrounds from biology to chemistry to physics. The book also contains useful step-by-step guides to building, modifying, and aligning TIRF microscopy systems for specialized purposes.
Key Features:
- Suitable for both basic and advanced levels
- Offers combinations with related techniques
- Relationship of evanescent effects between excitation and emission explained
- Practical guide to the optics as well
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