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Structural Genomics: General Applications
Paperback
Series: Methods in Molecular Biology, Book 2199
Medical ReferenceChemistryGenetics
ISBN13: 9781071608944
Publisher: Humana
Published: Nov 1 2021
Pages: 364
Weight: 1.45
Height: 0.78 Width: 7.00 Depth: 10.00
Language: English
Part I: Protein Production
1. High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis
Yuk Kei Wan, Gigi C.G. Choi, and Alan S.L. Wong
2. Rational Design and Construction of Active-Site Labeled Enzymes
Man-Wah Tsang, Yun-Chung Leung, and Kwok-Yin Wong
3. Screening and Production of Recombinant Human Proteins: Ligation Independent Cloning
Claire Strain-Damerell, Pravin Mahajan, Alejandra Fernandez-Cid, Opher Gileadi, and Nicola Burgess-Brown
4. Screening and Production of Recombinant Human Proteins: Protein Production in E. coli
Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Alejandra Fernandez-Cid, Opher Gileadi, and Susanne Gräslund
5. Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells
Pravin Mahajan, Claire Strain-Damerell, Shubhashish Mukhopadhyay, Alejandra Fernandez-Cid, Opher Gileadi, and Nicola A. Burgess-Brown
6. Expression Screening of Human Integral Membrane Proteins Using BacMam
Pravin Mahajan, Katherine Ellis, Shubhashish Mukhopadhyay, Alejandra Fernandez-Cid, Gamma Chi, Henry Man, Katharina L. Dürr, and Nicola A. Burgess-Brown
7. High-Throughput Expression Screening in Mammalian Suspension Cells
Susan D. Chapple and Michael R. Dyson
8. In Vitro Production of Perdeuterated Proteins in H2O for Biomolecular NMR Studies
Lionel Imbert, Rachel Lenoir-Capello, Elodie Crublet, Alicia Vallet, Rida Awad, Isabel Ayala, Celine Juillan-Binard, Hubert Mayerhofer, Rime Kerfah, Pierre Gans, Emeric Miclet, and Jerome Boisbouvier
9. Minimizing Heterogeneity of Protein Samples for Metal Transporter Proteins Using SAXS and Metal Radioisotopes
Shah Kamranur Rahman
Part II: Structural Analyses and Data Management
10. Hydrogen/Deuterium Exchange-Mass Spectrometry for Probing Changes in Conformation and Dynamics of Proteins
Pui-Kin So
11. BeStSel: From Secondary Structure Analysis to Protein Fold Prediction by Circular Dichroism Spectroscopy
András Micsonai, Éva Bulyáki, and József Kardos
12. Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb
Andrei L. Turinsky, Sam Dupont, Alexander Botzki, Sabry Razick, Brian Turner,
Ian M. Donaldson, and Shoshana J. Wodak
13. State-of-the-Art Data Management: Improving the Reproducibility, Consistency, and Traceability of Structural Biology and In Vitro Biochemical Experiments
David Cooper, Marek Grabowski, Matthew D. Zimmerman, Przemyslaw J. Porebski, Ivan G. Shabalin, Magdalena Woinska, Marcin J. Domagalski, Heping Zheng, Piotr Sroka, Marcin Cymborowski, Mateusz P. Czub, Ewa Niedzial
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