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Single-Cell Analysis: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 853
ISBN13: 9781493959211
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 238
Weight: 0.97
Height: 0.53 Width: 7.00 Depth: 10.00
Language: English
1. Introduction: Why Analyze Single Cells?
Dino Di Carlo, Henry Tat Kwong Tse, and Daniel R. Gossett
Part I: Conventional Methods
2. Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview
Sara Lindström
3. Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry
Robert B. Brown, Johannes A. Hewel, Andrew Emili, and Julie Audet
4. Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts
Ward De Spiegelaere, Muriel Filliers, and Ann Van Soom
Part II: Miniaturized Solutions
5. Single-Cell Culture in Microwells
Sara Lindström and Helene Andersson-Svahn
6. Iso-Dielectric Separation and Analysis of Cells
Michael D. Vahey and Joel Voldman
7. Single Cell Electroporation Using Microfluidic Devices
Séverine Le Gac and Albert van den Berg
8. Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array
Chi Zhang
9. Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay
Sara Henriksson and Mats Nilsson
10. Droplet Microfluidics for Single-Cell Analysis
Eric Brouzes
11. Screening of Antigen-Specific Antibody Secreting Cells
Hiroyuki Kishi, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, and Atsushi Muraguchi
12. Analysis of Single Eukaryotic Cells Using Raman Tweezers
Elsa Correia Faria and Peter Gardner
13. Single-Cell Microinjection Technologies
Yan Zhang
14. Ultrasonic Manipulation of Single Cells
Martin Wiklund and Björn Önfelt
Part III: Reviews on Chosen Subjects Within the Field
15. Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices
Soo Hyeon Kim, Dominique Fourmy, and Teruo Fujii
16. Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses
Ayça Yalçın, Yvonne J. Yamanaka, and J. Christopher Love
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