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Retinal Development: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 884
ISBN13: 9781493962525
Publisher: Humana
Published: May 4 2017
Pages: 367
Weight: 1.46
Height: 0.79 Width: 7.00 Depth: 10.00
Language: English
Part I: Manipulation of Gene Expression in vivo
1. Conditional Control of Gene Expression in the Mouse Retina
Qian Ding and Lin Gan
2. Generation Transgenic Xenopus using Restriction Enzyme-Mediated Intergration
Mohammad Haeri and Barry E. Knox
3. In vivo Functional Analysis of Transcription Factor--response Element Interaction using Transgenic Xenopus laevis
Heithem M. El-Hodiri, Yi Pan, and Lisa E. Kelly
4. Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse Eyes
John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Johnson, Lennart Berglin, T. Michael Redmond, Jeffrey H. Boatright
5. In Ovo Eye Electroporation
Teri L. Belecky-Adams, Scott R. Hudson, and Sarika Tiwari
6. Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in Xenopus Embryos
Sally A. Moody
Also see Chapter 8.
Part II: Tracing Cell Fate
7. Testing Retina Fate Commitment in Xenopus by Blastomere Deletion, Transplantation and Explant Culture
Sally A. Moody
8. Application of Cre-loxP Recombination for Lineage Tracing of Adult Zebrafish Retinal Stem Cells
Rajesh Ramachandran, Aaron Reifler, Jin Wan, and Daniel Goldman
9. Fate Tracing of neurogenin2-expressing Cells in the Mouse Retina using CreERTM - LacZ
Wenxin Ma and Shu-Zhen Wang
Part III: In vitro Systems
10. In Vitro Explant Culture and Related Protocols for the Study of Mouse Retinal Development
Kangxin Jin and Mengqing Xiang
11. In vitro Biochemical Assays to Monitor Rhodopsin Function
Joshua Sammons and Alecia K. Gross
12. Transfection of Primary Embryonic Chicken Retinal Cells using Cationic Lipid
Yi-Wen Hsieh and Xian-Jie Yang
13. Production of High Titer RCAS Retrovirus
Run-Tao Yan and Shu-Zhen Wang
14. Chick RPE Transdifferentiation Assay for Proneural Activities
Shu-Zhen Wang and Run-Tao Yan
Part IV: Regeneration/Stem Cells
15. Studying the Generation of Regenerated Retinal Neuron from Müller glia in the Mouse Eye
Mike O. Karl and Thomas A. Reh
16. Production and Transplantation of Retinal Cells from Human and Mouse Embryonic Stem Cells
Anna La Torre, Deepak A Lamba, Anu Jayabalu, Thomas A Reh
17. Light-induced Photoreceptor Degeneration in the Retina of the Zebrafish
Scott Taylor, Jing Chen, Jing Luo and Peter Hitchcock
18. Microarray-based Gene Profiling Analysis of Müller Glia-derived Retinal Stem Cells in Light-damaged Retinas from Adult Zebrafish
Zhao Qin and Pamela A. Raymond
Also see Chapter 8.
Part V: Function/Imaging
19: Measuring Rodent Electroretinograms to Assess Retinal Function
Molly E. Clark and Timothy W. Kraft
20. Functional Imaging of Retinal Photoreceptors and Inner Neurons using Stimulus-evoked Intrinsic Optical Signals
Xin-Cheng Yao and Yi-Chao Li
Part VI: Emerging Methodologies
21. Use of Laser Capture Microdissection for Analysis of Retinal mRNA/miRNA Expression and DNA Methylation
Laszlo Hackler Jr, Tomohiro Masuda, Verity F. Oliver, Shannath L. Merbs, and Donald J.Zack 22. Revealing Looping Organization of Mammalian Photoreceptor Genes Using Chromosome Conformation Capture (3C) Assays Guang-Hua Peng and Shiming Chen 23. Retinal Transcriptome Profiling by Directional Next Generation Sequencing Using 100 ng of Total RNA Matthew John Brooks, Harsha Karur Rajasimha, and Anand Swaroop 24. Exome Sequencing: Capture and Sequencing of All Human Coding Regions for Disease Gene Discovery Rinki Ratna Priya, Harsha Karur Rajasimha, Matthew Brooks, and Anand Swaroop 25. Determinati
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