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Recombinant Gene Expression
Hardcover
Series: Methods in Molecular Biology, Book 824
ISBN13: 9781617794322
Publisher: Humana
Published: Dec 13 2011
Pages: 649
Weight: 2.90
Height: 2.10 Width: 7.60 Depth: 10.40
Language: English
Table of Contents
Recombinant Gene Expression: Reviews and Protocols, Third Edition
Argelia Lorence (Editor)
Name of corresponding author(s) is underlined
General Aspects
1) Using Folding Promoting Agents in Recombinant Protein Production: A Review
Beatrix Fahnert
2) Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry
Brett J. Savary and Prasanna Vasu
3) A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression
MarÃa de la Cruz Muñoz-Centeno, Gonzalo Millán-Zambrano and Sebastián Chavez
4) Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins
Maureen C. Dolan, Giuliana Medrano, Anthony McMickle, and Carole L. Cramer
Prokaryotes
5) Heat-shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli
Christos A. Kyratsous and Christos A. Panagiotidis
6) The use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella
Ferenc Vonderviszt, Rachel Sajo, Jozsef Dobo, Peter Zavodoszky
7) Optimization of Purification Protocols Based on the Step-by-step Monitoring of the Protein Aggregates in the Soluble Fractions
Ario De Marco
8) Heterologous Protein Expression by Lactococcus lactis
Julio Villatoro-Hernández, Oscar Paul Kuipers, Odila Saucedo-Cárdenas and Roberto Montes de Oca Luna
9) An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview
Monserrat Argandoña, Carmen Vargas, Javier RodrÃguez-Moya, Mercedes Reina-Bueno, Manuel Salvador and Joaquin J. Nieto
10) Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia Pezzella, Ermenegilda Parrilli, Maria Luisa Tutino, Gennaro Marino, and Angela Duilio
11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella, Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino
12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein Interaction
Jumei Zeng and Zheng-Guo He
13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus
Nadine Katzke, René Bergmann, Karl-Erich Jaeger Thomas Drepper
14) Plasmid DNA Production for Therapeutic Applications
Alvaro Lara and Octavio T. Ramirez
Lower eukaryotes
15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review
Tomoaki Niimi
16) Expression of Multi-subunit Proteins in Leishmania tarentolae
Marisa Sugino and Tomoaki Niimi
Fungi
17) Recombinant Protein Production in Yeasts
Diethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro
18) Yeasts as Tool for Heterologous Gene Expression
Raja Mokdad-Gargouri, Salma Abdelmoula-Soussi, Nadia Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri
19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes
Simona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl
20) Aptamer-regulated Expression
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