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RNA Remodeling Proteins: Methods and Protocols

RNA Remodeling Proteins: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 3081

Biology

ISBN10: 1071655701
ISBN13: 9781071655702
Publisher: Humana
Published: Oct 13 2026
Pages: 532
Weight: 2.54
Height: 1.19 Width: 7.00 Depth: 10.00
Language: English
This third edition provides new and updated methods for the further study of RNA remodeling proteins. Chapters detail RNA remodeling proteins, AlphaFold to RNA-protein complexes, deep sequencing to probe transcriptome-wide remodeling events, helicase-dependent RNA structural changes, identifying helicase substrates, mapping transcriptional R-loops, leveraging techniques such as RNA-seq, DMS-seq, CLIP-seq, H-CRAC, Helicase-SELEX, proximity ligation, yeast three-hybrid assays, biochemical assays to measure RNA-protein affinity and helicase activities, structural and biophysical methods, an d functional characterization of RNA remodeling proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

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Biology