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RNA Detection and Visualization: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 714
ISBN13: 9781617790041
Publisher: Springer Nature
Published: Apr 6 2011
Pages: 497
Weight: 2.40
Height: 1.40 Width: 7.10 Depth: 10.10
Language: English
Part I: Visualizing mRNAs In situ
1. Single Molecule Imaging of RNA In situ
Mona Batish, Arjun Raj and Sanjay Tyagi
2. FISH and Immunofluorescence Staining in Chlamydomonas
James Uniacke, Daniel Colón-Ramos, and William Zerges
3. High Resolution Fluorescent In situ Hybridization in Drosophila
Eric Lécuyer
4. Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Using High Resolution In situ Hybridization
Alexandre Paix, Janet Chenevert, and Christian Sardet
5. Visualization of mRNA Localization in Xenopus Oocytes
James A. Gagnon and Kimberly L. Mowry
6. Visualization of mRNA Expression in the Zebrafish Embryo
Yossy Machluf and Gil Levkowitz
7. High-Resolution Fluorescence In situ Hybridization to Detect mRNAs in Neuronal Compartments In vitro and In vivo
Sharon A. Swanger, Gary J. Bassell, and Christina Gross
8. Localization of mRNA in Vertebrate Axons Using In situ Hybridization
José Roberto Sotelo-Silveira, Aldo Calliari, Alejandra Kun, Victoria Elizondo, LucÃa Canclini, and José Roberto Sotelo
Part II: Visualizing mRNAs In vivo Using Molecular Probes or Reconstituted Fluorescent Proteins
9. Tiny Molecular Beacons for In vivo mRNA Detection
Diana P. Bratu, Irina E. Catrina, and Salvatore A.E. Marras
10. Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA
Antony K. Chen, Won Jong Rhee, Gang Bao, and Andrew Tsourkas
11. Genetically-Encoded Fluorescent Probes for Imaging Endogenous mRNA in Living Cells
Takeaki Ozawa and Yoshio Umezawa
12. Visualization of Induced RNA in Single Bacterial Cells
Azra Borogovac and Natalia E. Broude
Part III: Visualizing mRNAs In vivo Using Aptamers and Intact Fluorescent Proteins
13. Visualizing mRNAs in Fixed and Living Yeast Cells
Franck Gallardo and Pascal Chartrand
14. In vivo Visualization of RNA Using the U1A-Based Tagged RNA System
Sunglan Chung and Peter A. Takizawa
15. Visualizing Endogenous mRNAs in Living Yeast Using m-TAG, a PCR-Based RNA Aptamer Integration Method, and Fluorescence Microscopy
Liora Haim-Vilmovsky and Jeffrey. E. Gerst
16. Imaging mRNAs in Living Mammalian Cells
Sharon Yunger and Yaron Shav-Tal
17. Using the mRNA-MS2/MS2CP-FP System to Study mRNA Transport during Drosophila Oogenesis
Katsiaryna Belaya and Daniel St. Johnston
Part IV: Use of Cell Fractionation to Demonstrate the Sub-Cellular Localization of RNA
18. Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria
Erez Eliyahu, Daniel Melamed, and Yoav Arava
19. Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation
Sujatha Jagannathan, Christine Nwosu, and Christopher V. Nicchitta
20. Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and Affinity Purification Procedure
Gadi Zipor, Cecile Brocard, and Jeffrey. E. Gerst
21. Profiling Axonal mRNA Transport
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