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RNA Detection: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1649
Please select the version of book you would like to purchase.
ISBN10: 149397212X
ISBN13: 9781493972128
Publisher: Humana Pr
Published: Nov 15 2017
Pages: 492
Weight: 2.41
Height: 1.13 Width: 7.00 Depth: 10.00
Language: English
ISBN13: 9781493972128
Publisher: Humana Pr
Published: Nov 15 2017
Pages: 492
Weight: 2.41
Height: 1.13 Width: 7.00 Depth: 10.00
Language: English
Preface...
Table of Contents...
Contributing Authors...
1. The Secret Life of RNA: Lessons from Emerging Methodologies
Caroline Medioni and Florence Besse
2. Quantification of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
Lilia Ayadi, Yuri Motorin, and Virginie Marchand 3. Identifying the m6A Methylome by Affinity Purification and Sequencing
Phillip J. Hsu and Chuan He 4. PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
Zhipeng Lu, Jing Gong, and Qiangfeng Cliff Zhang 5. Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
Toshiaki Shigeoka, Jane Jung, Christine E. Holt, and Hosung Jung 6. LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
Susanne Nichterwitz, Julio Aguila Benitez, Rein Hoogstraaten, Qiaolin Deng, and Eva Hedlund 7. Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
Kaia Achim, Hernando MartÃnez Vergara, and Jean-Baptiste Pettit 8. Single mRNA Molecule Detection in Drosophila
Shawn C. Little and Thomas Gregor 9. Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
L. Carine Stapel, Coleman Broaddus, and Nadine L. Vastenhouw 10. Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
Joshua S. Titlow, Lu Yang, Richard M. Parton, Ana Palanca, and Ilan Davis 11. Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
Catherine Rabouille 12. Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification
Yao Xu and Zhi Zheng 13. In Situ Detection of MicroRNA Expression with RNAscope Probes
Viravuth P. Yin 14. Padlock Probes to Detect Single Nucleotide Polymorphisms
Tomasz Krzywkowski and Mats Nilsson 15. Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
Yantao Yang, Mingming Chen, Christopher K. Krueger, Andrew Tsourkas, and Antony K. Chen 16. Optimizing Molecular Beacons for Intracellular Analysis of RNA
Mingming Chen, Yantao Yang, Christopher K. Krueger, and Antony K. Chen 17. Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
Dan Ohtan Wang 18. In Vivo Visualization and Function Probing of Transport mRNPs using Injected FIT Probes
Jasmine Chamiolo, Imre Gaspar, Anne Ephrussi, and Oliver Seitz 19. Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers
Murat Sunbul, Ankita Arora, and Andres Jäschke 20. Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe
Shin-ichi Sato, Kenji Yatsuzuka, Yousuke Katsuda, and Motonari Uesugi 21. RNA Live Imaging in the Model Microorganism Ustilago maydis
S. Zander, K. Müntjes, and M. Feldbrügge 22. Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells
Hideaki Yoshimura and Takeaki Ozawa 23. Live Imaging of mRNA Synthesis in Drosophila
Hernan G. Garcia and Thomas Gregor 24. Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
Anupam A. Sawan
Phillip J. Hsu and Chuan He 4. PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
Zhipeng Lu, Jing Gong, and Qiangfeng Cliff Zhang 5. Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
Toshiaki Shigeoka, Jane Jung, Christine E. Holt, and Hosung Jung 6. LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
Susanne Nichterwitz, Julio Aguila Benitez, Rein Hoogstraaten, Qiaolin Deng, and Eva Hedlund 7. Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
Kaia Achim, Hernando MartÃnez Vergara, and Jean-Baptiste Pettit 8. Single mRNA Molecule Detection in Drosophila
Shawn C. Little and Thomas Gregor 9. Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
L. Carine Stapel, Coleman Broaddus, and Nadine L. Vastenhouw 10. Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
Joshua S. Titlow, Lu Yang, Richard M. Parton, Ana Palanca, and Ilan Davis 11. Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
Catherine Rabouille 12. Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification
Yao Xu and Zhi Zheng 13. In Situ Detection of MicroRNA Expression with RNAscope Probes
Viravuth P. Yin 14. Padlock Probes to Detect Single Nucleotide Polymorphisms
Tomasz Krzywkowski and Mats Nilsson 15. Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
Yantao Yang, Mingming Chen, Christopher K. Krueger, Andrew Tsourkas, and Antony K. Chen 16. Optimizing Molecular Beacons for Intracellular Analysis of RNA
Mingming Chen, Yantao Yang, Christopher K. Krueger, and Antony K. Chen 17. Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
Dan Ohtan Wang 18. In Vivo Visualization and Function Probing of Transport mRNPs using Injected FIT Probes
Jasmine Chamiolo, Imre Gaspar, Anne Ephrussi, and Oliver Seitz 19. Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers
Murat Sunbul, Ankita Arora, and Andres Jäschke 20. Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe
Shin-ichi Sato, Kenji Yatsuzuka, Yousuke Katsuda, and Motonari Uesugi 21. RNA Live Imaging in the Model Microorganism Ustilago maydis
S. Zander, K. Müntjes, and M. Feldbrügge 22. Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells
Hideaki Yoshimura and Takeaki Ozawa 23. Live Imaging of mRNA Synthesis in Drosophila
Hernan G. Garcia and Thomas Gregor 24. Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
Anupam A. Sawan
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