Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Resin Microscopy and On-Section Immunocytochemistry

Resin Microscopy and On-Section Immunocytochemistry

Newman, Geoffrey R.
Hobot, Jan A.

Paperback

Series: Springer Lab Manuals

Medical ReferenceGeneral Science

ISBN10: 354067277X
ISBN13: 9783540672777
Publisher: Springer Nature
Published: Apr 10 2001
Pages: 273
Weight: 1.13
Height: 0.62 Width: 7.50 Depth: 9.25
Language: English
Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for on-section cytochemistry and immunocytochemistry are described, including detailed protocols for the application.

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