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Receptor and Ion Channel Detection in the Brain
Paperback
Series: Neuromethods, Book 169
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ISBN10: 1071615246
ISBN13: 9781071615249
Publisher: Humana
Published: Jul 28 2022
Pages: 554
Weight: 2.17
Height: 1.17 Width: 7.00 Depth: 10.00
Language: English
ISBN13: 9781071615249
Publisher: Humana
Published: Jul 28 2022
Pages: 554
Weight: 2.17
Height: 1.17 Width: 7.00 Depth: 10.00
Language: English
Series Preface...
Preface...
Table of Contents...
Contributing Authors...
Masahiko Watanabe 2. Co-Immunoprecipitation from Brain
Xavier Morató, Dasiel O. Borroto-Escuela, Kjell Fuxe, Víctor Fernández-Dueñas, and Francisco Ciruela 3. Sub-Synaptic Membrane Fractionation
Paula M. Canas and Rodrigo A. Cunha 4. Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation
Elek Molnár 5. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells
Juan Varela, Julien Dupuis, François Maingret, and Laurent Groc 6. Radioligand Binding Detection of Receptors in Brain Membranes
Fuencisla Pilar-Cuéllar, Emilio Garro-Martínez, Elena Castro, and Álvaro Díaz 7. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain
Markus U. Ehrengruber and Kenneth Lundstrom 8. Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain
Víctor Fernández-Dueñas, Thierry Durroux, and Francisco Ciruela 9. Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay
Manuel Narváez, Minerva Crespo-Ramírez, Ramón Fores-Pons, Mariana Pita-Rodríguez, Francisco Ciruela, Malgorzata Filip, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Miguel Perezde la Mora, Kjell Fuxe, and Dasiel O. Borroto-Escuela 10. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain
Marta Valle-León, Víctor Fernández-Dueñas, and Francisco Ciruela Part II Neuroanatomical Techniques 11. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling
Miwako Yamasaki and Masahiko Watanabe 12. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain
Rebeca Vidal, Fuencisla Pilar-Cuellar, Álvaro Díaz, and Elena Castro 13. Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting
Elek Molnár 14. Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility
Kohtarou Konno and Masahiko Watanabe 15. Localization of GFP-Tagged Proteins at the Electron Microscope
Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil, J.S. Lee, M.V. Nachury, and José Manuel García-Verdugo 16. Tyramide Signal Amplificación for Immunoelectron Microscopy
María José Ulloa-Navas, Patricia García-Tárraga, Susana González-Granero, Pedro Pérez-Borreda, Vicente Herranz-Pérez, and José Manuel García-Verdugo 17. Pre-Embedding Methods for the Localization of Receptors and Ion Channels
Rafael Luján 18. Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels
Rafael Luján and Masahiko Watanabe 19. High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)
Walter A. Kaufmann, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto 20. Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM
Marta Turégano-López, José Rodrigo-Rodríguez, Lidia Alonso-Nanclares, Juncal González-Soriano, Javier DeFelipe, and Ángel Merchán-Pérez 21. Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies
Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta 22. Efficient Labeling of Neurons and Identification of P
Elek Molnár 5. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells
Juan Varela, Julien Dupuis, François Maingret, and Laurent Groc 6. Radioligand Binding Detection of Receptors in Brain Membranes
Fuencisla Pilar-Cuéllar, Emilio Garro-Martínez, Elena Castro, and Álvaro Díaz 7. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain
Markus U. Ehrengruber and Kenneth Lundstrom 8. Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain
Víctor Fernández-Dueñas, Thierry Durroux, and Francisco Ciruela 9. Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay
Manuel Narváez, Minerva Crespo-Ramírez, Ramón Fores-Pons, Mariana Pita-Rodríguez, Francisco Ciruela, Malgorzata Filip, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Miguel Perezde la Mora, Kjell Fuxe, and Dasiel O. Borroto-Escuela 10. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain
Marta Valle-León, Víctor Fernández-Dueñas, and Francisco Ciruela Part II Neuroanatomical Techniques 11. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling
Miwako Yamasaki and Masahiko Watanabe 12. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain
Rebeca Vidal, Fuencisla Pilar-Cuellar, Álvaro Díaz, and Elena Castro 13. Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting
Elek Molnár 14. Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility
Kohtarou Konno and Masahiko Watanabe 15. Localization of GFP-Tagged Proteins at the Electron Microscope
Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil, J.S. Lee, M.V. Nachury, and José Manuel García-Verdugo 16. Tyramide Signal Amplificación for Immunoelectron Microscopy
María José Ulloa-Navas, Patricia García-Tárraga, Susana González-Granero, Pedro Pérez-Borreda, Vicente Herranz-Pérez, and José Manuel García-Verdugo 17. Pre-Embedding Methods for the Localization of Receptors and Ion Channels
Rafael Luján 18. Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels
Rafael Luján and Masahiko Watanabe 19. High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)
Walter A. Kaufmann, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto 20. Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM
Marta Turégano-López, José Rodrigo-Rodríguez, Lidia Alonso-Nanclares, Juncal González-Soriano, Javier DeFelipe, and Ángel Merchán-Pérez 21. Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies
Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta 22. Efficient Labeling of Neurons and Identification of P
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